Regulation and properties of KCNQ1 (K(v)LQT1) and impact of the cystic fibrosis transmembrane conductance regulator

Boucherot, A., Schreiber, R. and Kunzelmann, K. (2001) Regulation and properties of KCNQ1 (K(v)LQT1) and impact of the cystic fibrosis transmembrane conductance regulator. Journal of Membrane Biology, 182 1: 39-47.


Author Boucherot, A.
Schreiber, R.
Kunzelmann, K.
Title Regulation and properties of KCNQ1 (K(v)LQT1) and impact of the cystic fibrosis transmembrane conductance regulator
Journal name Journal of Membrane Biology   Check publisher's open access policy
ISSN 0022-2631
Publication date 2001-07
Sub-type Article (original research)
Volume 182
Issue 1
Start page 39
End page 47
Total pages 9
Place of publication United States
Publisher Springer Verlag
Collection year 2001
Language eng
Subject C1
270104 Membrane Biology
730110 Respiratory system and diseases (incl. asthma)
1108 Medical Microbiology
Abstract The K+ channel KCNQ1 (K(V)LQT1) is a voltage-gated K+ channel, coexpressed with regulatory subunits such as KCNE1 (IsK, mink) or KCNE3, depending on the tissue examined. Here, we investigate regulation and properties of human and rat KCNQ1 and the impact of regulators such as KCNE1 and KCNE3. Because the cystic fibrosis transmembrane conductance regulator (CFTR) has also been suggested to regulate KCNQ1 channels we studied the effects of CFTR on KCNQ1 in Xenopus oocytes, Expression of both human and rat KCNQ1 induced time dependent K+ currents that were sensitive to Ba2+ and 293B. Coexpression with KCNE1 delayed voltage activation, while coexpression with KCNE3 accelerated current activation. KCNQ1 currents were activated by an increase in intracellular cAMP, independent of coexpression with KCNE1 or KCNE3. cAMP dependent activation was abolished in N-terminal truncated hKCNQ1 but was still detectable after deletion of a single PKA phosphorylation motif. In the presence but not in the absence of KCNE1 or KCNE3, K+ currents were activated by the Ca2+ ionophore ionomycin. Coexpression of CFTR with either human or rat KCNQ1 had no impact on regulation of KCNQ1 K+ currents by cAMP but slightly shifted the concentration response curve for 293B. Thus, KCNQ1 expressed in Xenopus oocytes is regulated by cAMP and Ca2+ but is not affected by CFTR.
Keyword Biochemistry & Molecular Biology
Cell Biology
Physiology
K(v)lqt1
Kcnq1
Kcne1
Isk
Mink
Kcne3
Rat Colon
K+ Channel
Electrolyte Secretion
Xenopus Oocytes
293b
Camp
Potassium Channel
Cl Secretion
K+ Conductance
Cftr
Epithelia
Proteins
Domain
Form
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 14 Aug 2007, 15:11:30 EST