Localization of neurotransmitters and calcium binding proteins to neurons of salamander and mudpuppy retinas

Deng, Ping, Cuenca, Nicolas, Doerr, Terry, Pow, David V., Miller, Robert and Kolb, Helga (2001) Localization of neurotransmitters and calcium binding proteins to neurons of salamander and mudpuppy retinas. Vision Research, 41 14: 1771-1783. doi:10.1016/S0042-6989(01)00060-8


Author Deng, Ping
Cuenca, Nicolas
Doerr, Terry
Pow, David V.
Miller, Robert
Kolb, Helga
Title Localization of neurotransmitters and calcium binding proteins to neurons of salamander and mudpuppy retinas
Journal name Vision Research   Check publisher's open access policy
ISSN 0042-6989
1878-5646
Publication date 2001-06
Sub-type Article (original research)
DOI 10.1016/S0042-6989(01)00060-8
Volume 41
Issue 14
Start page 1771
End page 1783
Total pages 13
Editor H. Spekreijse
W. Baehr
S.M. Wu
R. Blake
Place of publication Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2001
Language eng
Subject C1
270107 Cell Neurochemistry
780108 Behavioural and cognitive sciences
Abstract We wished to identify the different types of retinal neurons on the basis of their content of neuroactive substances in both larval tiger salamander and mudpuppy retinas, favored species for electrophysiological investigation. Sections and wholemounts of retinas were labeled by immunocytochemical methods to demonstrate three calcium binding protein species and the common neurotransmitters, glycine, GABA and acetylcholine. Double immunostained sections and single labeled wholemount retinas were examined by confocal microscopy. Immunostaining patterns appeared to be the same in salamander and mudpuppy. Double and single cones, horizontal cells, some amacrine cells and ganglion cells were strongly calbindin-immunoreactive (IR). Calbindin-IR horizontal cells colocalized GABA. Many bipolar cells, horizontal cells, some amacrine cells and ganglion cells were strongly calretinin-IR. One type of horizontal cell and an infrequently occurring amacrine cell were parvalbumin-IR. Acetylcholine as visualized by ChAT-immunoreactivity was seen in a mirror-symmetric pair of amacrine cells that colocalized GABA and glycine. Glycine and GABA colocalized with calretinin, calbindin and occasionally with parvalbumin in amacrine cells. (C) 2001 Elsevier Science Ltd. All rights reserved.
Keyword Neurosciences
Ophthalmology
Calretinin
Calbindin
Parvalbumin
Chat
Glycine
Gaba
Confocal Microscopy
Larval Tiger Salamander
Gamma-aminobutyric-acid
Immunoreactive Amacrine Cells
Ganglion-cells
Bipolar Cells
Cat Retina
Mammalian Retina
Glutamate Immunoreactivity
Pharmacological Basis
Vertebrate Retina
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Physical Sciences Publications
 
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Created: Tue, 14 Aug 2007, 15:06:40 EST