Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell

Lindsay, Margaret R., Webb, Richard I., Strous, Marc, Jetten, Mike S. M., Butler, Margaret K., Forde, Rebecca J. and Fuerst, John A. (2001) Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell. Archives of Microbiology, 175 6: 413-429. doi:10.1007/s002030100280


Author Lindsay, Margaret R.
Webb, Richard I.
Strous, Marc
Jetten, Mike S. M.
Butler, Margaret K.
Forde, Rebecca J.
Fuerst, John A.
Title Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell
Journal name Archives of Microbiology   Check publisher's open access policy
ISSN 0302-8933
Publication date 2001
Sub-type Article (original research)
DOI 10.1007/s002030100280
Volume 175
Issue 6
Start page 413
End page 429
Total pages 17
Place of publication New York
Publisher Springer-Verlag
Collection year 2001
Language eng
Subject C1
270301 Bacteriology
780105 Biological sciences
Abstract The organisation of cells of the planctomycete species Pirellula marina, Isosphaera pallida, Gemmata obscuriglobus, Planctomyces mat-is and Candidatus Brocadia anammoxidans was investigated based on ultrastructure derived from thin-sections of cryosubstituted cells, freeze-fracture replicas, and in the case of Gemmata obscuriglobus and Pirellllla marina, computer-aided 3-D reconstructions from serial sections of cryosubstituted cells. All planctomycete cells display a peripheral ribosome-free region, termed here the paryphoplasm, surrounding the perimeter of the cell, and an interior region including any nucleoid regions as well as ribosome-like particles, bounded by a single intracytoplasmic membrane (ICM), and termed the pirellulosome in Pirellula species. Immunogold labelling and RNase-gold cytochemistry indicates that in planctomycetes all the cell DNA is contained wholly within the interior region bounded by the ICM, and the paryphoplasm contains no DNA but at least some of the cell's RNA. The ICM in Isosphaera pallida and Planctomyces mat-is is invaginated such that the paryphoplasm forms a major portion of the cell interior in sections, but in other planctomycetes it remains as a peripheral zone. In the anaerobic ammonium-oxidising (anammox process) chemoautotroph Candidatus Brocadia anammoxidans the interior region bounded by ICM contains a further internal single-membrane-bounded region, the anam-moxosome. In Gemmata obscuriglobus. the interior ICM-bounded region contains the nuclear body, a double-membrane-bounded region containing the cell's nucleoid and all genomic DNA in addition to some RNA. Shared features of cell compartmentalisation in different planctomycetes are consistent with the monophyletic nature of the planctomycetes as a distinct division of the Bacteria. The shared organisational plan for the planctomycete cell constitutes a new type not known in cells of other bacteria.
Keyword Microbiology
Bacteria
Planctomycetes
Planctomycetales
Ultrastructure
Cell Compartmentalisation
Nucleoids
Intracytoplasmic Membranes
Cryosubstitution
Freeze-fracture
Electron Microscopy
16s Ribosomal-rna
Gemmata-obscuriglobus
Order Planctomycetales
Budding Bacteria
Genera Planctomyces
Nov
Diversity
Pirella
Genus
Microorganisms
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 198 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 214 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 14:54:48 EST