Growth factors and cytokines modulate gene expression of cell-surface proteoglycans in human periodontal ligament cells

Worapamorn, W., Haase, H. R., Li, H. and Bartold, P. M. (2001) Growth factors and cytokines modulate gene expression of cell-surface proteoglycans in human periodontal ligament cells. Journal of Cellular Physiology, 186 3: 448-456. doi:10.1002/1097-4652(2001)9999:9999<000::AID-JCP1047>3.0.CO;2-V


Author Worapamorn, W.
Haase, H. R.
Li, H.
Bartold, P. M.
Title Growth factors and cytokines modulate gene expression of cell-surface proteoglycans in human periodontal ligament cells
Journal name Journal of Cellular Physiology   Check publisher's open access policy
ISSN 1097-4652
0021-9541
Publication date 2001
Sub-type Article (original research)
DOI 10.1002/1097-4652(2001)9999:9999<000::AID-JCP1047>3.0.CO;2-V
Volume 186
Issue 3
Start page 448
End page 456
Total pages 9
Editor C. Basilico
Place of publication USA
Publisher John Wiley & Sons Inc
Collection year 2001
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
Abstract Cell-surface proteoglycans have been known to be involved in many functions including interactions with components of the extracellular microenvironment, and act as co-receptors which bind and modify the action of various growth factors and cytokines. The purpose of this study was to determine the regulation by growth factors and cytokines on cell-surface proteoglycan gene expression in cultured human periodontal ligament (PDL) cells. Subconfluent, quiescent PDL cells were treated with various concentrations of serum, bFGF, PDGF-BB, TGF-beta1, IL-1 beta, and IFN-gamma. RT-PCR technique was used, complemented with Northern blot for syndecan-1, to examine the effects of these agents on the mRNA expression of five cell-surface proteoglycans (syndecan-1, syndecan-2, syndecan-4, glypican and betaglycan). Syndecan-1 mRNA levels increased in response to serum, bFGF and PDGF-BB, but decreased in response to TGF-beta1, IL-1 beta and IFN-gamma. In contrast, syndecan-2 mRNA levels were upregulated by TCF-beta1 and IL-1 beta stimulation, but remained unchanged with the other agents. Betaglycan gene expression decreased in response to serum, but was upregulated by TCF-beta1 and unchanged by the other stimulants. Additionally, syndecan-4 and glypican were not significantly altered in response to the regulator molecules studied, with the exception that glypican is decreased in response to IFN-gamma. These data demonstrate that the gene expression of the five cell-surface proteoglycans studied is differentially regulated in PDL cells lending support to the nation of distinct functions for these cell-surface proteoglycans. (C) 2001 Wiley-Liss, inc.
Keyword Cell Biology
Physiology
Human-lung Fibroblasts
Necrosis-factor-alpha
Smooth-muscle-cells
Human B-lymphocytes
Heparan-sulfate
Transforming Growth-factor-beta-1
Syndecan-1 Expression
Molecular-cloning
Binding
Differentiation
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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Created: Tue, 14 Aug 2007, 14:52:40 EST