Regional proton nuclear magnetic resonance spectroscopy differentiates cortex and medulla in the isolated perfused rat kidney

Cowin, G. J., Leditschke, I. A., Crozier, S., Brereton, I. M. and Endre, Z. H. (1997) Regional proton nuclear magnetic resonance spectroscopy differentiates cortex and medulla in the isolated perfused rat kidney. Magnetic Resonance Materials in Physics, Biology and Medicine, 5 2: 151-158. doi:10.1007/BF02592246


Author Cowin, G. J.
Leditschke, I. A.
Crozier, S.
Brereton, I. M.
Endre, Z. H.
Title Regional proton nuclear magnetic resonance spectroscopy differentiates cortex and medulla in the isolated perfused rat kidney
Journal name Magnetic Resonance Materials in Physics, Biology and Medicine   Check publisher's open access policy
ISSN 0968-5243
1352-8661
Publication date 1997-06
Sub-type Article (original research)
DOI 10.1007/BF02592246
Volume 5
Issue 2
Start page 151
End page 158
Total pages 8
Editor Patrick J. Cozzone
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Formatted abstract
Volume-localized proton nuclear magnetic resonance spectroscopy was used as an assay of regional biochemistry in the isolated perfused rat kidney. This model eliminated artifacts caused by respiratory and cardiac motion experienced in vivo. Immersion of the kidney under its venous effluent reduced the susceptibility artifacts evoked by tissue-air interfaces. The rapid acquisition with relaxation enhancement imaging sequence was used for scout imaging. This gave excellent spatial resolution of the cortex, outer medulla, and inner medulla. Spectra were then acquired in 10 minutes using the volume-selective multipulse spectroscopy sequence from voxels with a volume of approximately 24 μL located within the cortical or medullary regions. Spectral peaks were assigned by the addition of known compounds to the perfusion medium and by comparison with spectra of protein-free extracts of cortex and medulla. The medullary region spectra were characterized by signals from the osmolytes betaine, glycerophosphorylcholine, and inositol. The spectra from the cortex were more complex and contained lesser contributions from osmolytes.
© 1997 Chapman & Hall
Keyword Radiology, Nuclear Medicine & Medical Imaging
Localized Nuclear Magnetic Resonance Spectroscopy
Isolated perfused rat kidney
Renal osmolytes
Renal inner medulla
Organic osmolytes
NMR-spectroscopy
In-vivo
Echo
Dehydration
Responses
H-1-NMR
Tesla
MR
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Medicine Publications
Centre for Advanced Imaging Publications
 
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Created: Mon, 13 Aug 2007, 16:54:56 EST