Lipopolysaccharide biosynthesis genes in koala type I Chlamydia: Cloning and characterization

Girjes, AA, Carrick, FN and Lavin, MF (1997) Lipopolysaccharide biosynthesis genes in koala type I Chlamydia: Cloning and characterization. Research In Microbiology, 148 5: 413-425. doi:10.1016/S0923-2508(97)83872-7


Author Girjes, AA
Carrick, FN
Lavin, MF
Title Lipopolysaccharide biosynthesis genes in koala type I Chlamydia: Cloning and characterization
Journal name Research In Microbiology   Check publisher's open access policy
ISSN 0923-2508
Publication date 1997-01-01
Sub-type Article (original research)
DOI 10.1016/S0923-2508(97)83872-7
Volume 148
Issue 5
Start page 413
End page 425
Total pages 13
Language eng
Abstract We showed in 1988 that there are two strains of Chlamydia psittaci which infect the koala (Phascolarctos cinereus). In order to further investigate the role of these chlamydial strains in pathogenesis, we have attempted to identify genes of koala type I strain chlamydial which are involved in the immunogenic response, Transformation of Escherichia coli with a plasmid containing a 6.3-kb fragment (pKOC-10) of C. psittaci DNA caused the appearance of a specific chlamydial lipopolysaccharide (LPS) epitope on the host strain. The smallest DNA fragment capable of inducing the expression of chlamydial LPS was an Xbal fragment, 2.4 kb in size (pKOC-5). DNA sequence analysis of the complete fragment revealed regions of high identity, at the amino acid level, to the gseA genes of C. pneomoniae, C. psittaci 6BC and C. trachomatis, and the kdtA gene of E. coli which code for transferases catalysing the addition of 3-deoxy-D-manno-octulosonic acid (Kdo) residues to lipid A. Two open reading frames (ORFs) of 1,314 and 501 nucleotides in size, within the 2.4-kb fragment, were evident, and mRNA species corresponding to these ORFs were detected by Northern analysis. Both ORF1 and ORF2 are required for the appearance of chlamydia-specific LPS on the surface of recombinant E. coli.
Keyword Microbiology
Lps
Chlamydia
Epitope
Kdo
Gsea
3-deoxy-d-manno-octulosonic Acid Transferase
Polyacrylamide Gel-electrophoresis
Genus-specific Epitope
Escherichia-coli
Phascolarctos-cinereus
Psittaci Strains
Antigen
Trachomatis
Expression
Antibodies
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 14 Aug 2007, 02:54:07 EST