AP-1 and Egr DNA-binding activities are increased in rat brain during ethanol withdrawal

Beckmann, AM, Matsumoto, I and Wilce, PA (1997) AP-1 and Egr DNA-binding activities are increased in rat brain during ethanol withdrawal. Journal of Neurochemistry, 69 1: 306-314.

Author Beckmann, AM
Matsumoto, I
Wilce, PA
Title AP-1 and Egr DNA-binding activities are increased in rat brain during ethanol withdrawal
Journal name Journal of Neurochemistry   Check publisher's open access policy
ISSN 0022-3042
Publication date 1997
Sub-type Article (original research)
Volume 69
Issue 1
Start page 306
End page 314
Total pages 9
Language eng
Abstract The DNA-binding activities of AP-1 and Egr proteins were investigated in nuclear extracts of rat brain regions during ethanol withdrawal. Both DNA-binding activities were transiently elevated in the hippocampus and cerebellum 16 h after withdrawal. In the cerebral cortex, AP-1 and Egr DNA-binding activities increased at 16 h and persisted until 32 and 72 h, respectively. The AP-1 DNA-binding activities in all regions at all times after withdrawal were composed of FosB, c-Jun, JunB, and JunD. c-Fos was detected at all times in the cerebral cortex, at 16 h only in the hippocampus, and from 16 to 72 h in the cerebellum. Withdrawal severity did not affect the composition of the AP-1 DNA-binding activities. Two Egr DNA-binding activities were present in the cortex and hippocampus. The faster-migrating complex predominated in hippocampus, and only the slower-migrating complex (identified as Egr-1) was present in the cerebellum. The increase in DNA-binding activity of immediate early gene-encoded transcription factors supports their proposed role in initiating a cascade of altered gene expression underlying the long-term neuronal response to ethanol withdrawal.
Keyword Biochemistry & Molecular Biology
Neurosciences
C-fos
C-jun
Egr-1
Immediate Early Genes
Ethanol Withdrawal
Methyl-d-aspartate
Gamma-aminobutyric-acid
Transcription Factor
Electroconvulsive Seizure
Receptor Complex
Gene-expression
C-fos
Proteins
Family
Hippocampus
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Mon, 13 Aug 2007, 16:52:05 EST