Regulation of CSF-1 receptor expression

Hume, D. A., Yue, X., Ross, I. L., Favot, P., Lichanska, A. and Ostrowski, M. C. (1997) Regulation of CSF-1 receptor expression. Molecular Reproduction and Development, 46 1: 46-53. doi:10.1002/(SICI)1098-2795(199701)46:1<46::AID-MRD8>3.0.CO;2-R


Author Hume, D. A.
Yue, X.
Ross, I. L.
Favot, P.
Lichanska, A.
Ostrowski, M. C.
Title Regulation of CSF-1 receptor expression
Journal name Molecular Reproduction and Development   Check publisher's open access policy
ISSN 1040-452X
1098-2795
Publication date 1997
Sub-type Article (original research)
DOI 10.1002/(SICI)1098-2795(199701)46:1<46::AID-MRD8>3.0.CO;2-R
Volume 46
Issue 1
Start page 46
End page 53
Total pages 8
Place of publication John Wiley & Sons
Publisher Hoboken, NJ, U.S.A.
Language eng
Subject 0604 Genetics
Abstract Cells of the mononuclear phagocyte lineage possess receptors for macrophage colony-stimulating factor (CSF-1) encoded by the c-fms protooncogene and respond to CSF-1 with increased survival, growth, differentiation, and reversible changes in function. The c-fms gene is itself a macrophage differentiation marker. In whole mount analyses of mRNA expression in embryos, c-fms is expressed at very high levels on placental trophoblasts. It is detectable on individual cells in the yolk sac around 8.5 to 9 days postcoitus, appears on isolated cells in the head of the embryo around 9.5 dpc, and appears on numerous cells throughout the embryo by day 10.5. The extent of c-fms expression is much greater than for other macrophage-specific genes including lysozyme and a macrophage-specific protein tyrosine phosphatase. Our studies of the cis-acting elements of the c-fms promoter have indicated a key role for collaboration between the macrophage-specific transcription factor, Pu.1, which functions in determining the site of transcription initiation, and other members of the Ets transcription factor family. This is emerging as a common pattern in macrophage-specific promoters. We have shown that two PU box elements alone can function as a macrophage-specific promoter. The activity of both the artifical promoter and the c-fms promoter is activated synergistically by coexpression of Pu.1 and another Ets factor, c-Ets-2. A 3.5kb c-fms exon 2 promoter (but not the 300bp proximal promoter) is also active in a wide diversity of tumor cell lines. The interesting exception is the melanoma cell line K1735, in which the promoter is completely shut down and expression of c-fms causes growth arrest and cell death. The activity of the exon 2 promoter in these nonmacrophages is at least as serum responsive as the classic serum-responsive promoter of the c-fos gene. It is further inducible in nonmacrophages by coexpression of the c-fms product. Unlike other CSF-1/c-fms-responsive promoters, the c-fms promoter is not responsive to activated Ras even when c-Ets-2 is coexpressed. In most lines, production of full length c-fms is prevented by a downstream intronic terminator, but in Lewis lung carcinoma, read-through does occur, and expression of both c-fms and other macrophage-specific genes such as lysozyme and urokinase becomes detectable in conditions of serum deprivation. (C) 1997 Wiley-Liss, Inc.
Keyword CSF1R
Pu.1
Ets
c-fms
in situ
Macrophage
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Mon, 13 Aug 2007, 16:38:49 EST