XANES investigation of the Co oxidation state in solution and in cancer cells treated with Co(III) complexes

Bonnitcha, Paul D., Hall, Matthew D., Underwood, Clare K., Foran, Garry J., Zhang, Mei, Beale, Philip J. and Hambley, Trevor W. (2006) XANES investigation of the Co oxidation state in solution and in cancer cells treated with Co(III) complexes. Journal of Inorganic Biochemistry, 100 5-6: 963-971. doi:10.1016/j.jinorgbio.2006.02.015


Author Bonnitcha, Paul D.
Hall, Matthew D.
Underwood, Clare K.
Foran, Garry J.
Zhang, Mei
Beale, Philip J.
Hambley, Trevor W.
Title XANES investigation of the Co oxidation state in solution and in cancer cells treated with Co(III) complexes
Journal name Journal of Inorganic Biochemistry   Check publisher's open access policy
ISSN 0162-0134
1873-3344
Publication date 2006-05
Sub-type Article (original research)
DOI 10.1016/j.jinorgbio.2006.02.015
Volume 100
Issue 5-6
Start page 963
End page 971
Total pages 9
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract XANES spectroscopy has been used to investigate whether it is possible to determine the oxidation state and coordination environment of Co complexes following treatment of cancer cells with Co(III) or Co(II) complexes. Our results show that the variation of the XANES with coordination geometry make it impossible to do this in a completely reliable way which is in contrast to the situation for platinum and chromium. It was established that the XANES spectrum obtained from cells treated with [Co(diNOsar)]Br3 remained unchanged with respect to its XANES spectrum obtained in solution, demonstrating that the [Co(diNOsar)]Br3 complex remained intact after 24 h in cellular media (diNOsar = 1,8-dinitro-3,6,10,13,16,19-hexaazabicyclo[6.6.6]eicosane). In contrast, the XANES spectra obtained from cells treated with Na[Co(acac)3] and [Co(acac)3] differed from the XANES spectra of the respective complexes obtained in solution, indicating a change in co-ordination environment for both complexes upon uptake in cells. The similarity of these spectra suggests that appearance of this XANES can be used as an indication of loss of the carrier ligands, a useful indicator in the study of hypoxia selective complexes. The results obtained for Na[Co(acac)3] and [Co(acac)3] are consistent with the intracellular coordination of cobalt(III) to sulfur ligands upon cellular uptake.
Keyword Cobalt
Hypoxia
XANES
Cancer
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Special Issue Containing Contributions from the 12th International Conference on Biological Inorganic Chemistry

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Mon, 13 Aug 2007, 16:16:32 EST