Functional and molecular alterations to cell cycle are seen in biopsied olfactory neuroepithelium from patients with schizophrenia and bipolar I disorder

McCurdy, R. D., Feron, F., Perry, C., Chant, D. C., McLean, D., Matigian, N., Hayward, N. K., McGrath, J. J. and Mackay-Sim, A. (2006). Functional and molecular alterations to cell cycle are seen in biopsied olfactory neuroepithelium from patients with schizophrenia and bipolar I disorder. In: Schizophrenia Research : Abstracts of the XIIIth Biennial Winter Workshop on Schizophrenia Research. XIIIth Biennial Winter Workshop on Schizophrenia Research, Davos, Switzerland, (198-198). 4-10 February, 2006. doi:10.1016/j.schres.2006.01.006


Author McCurdy, R. D.
Feron, F.
Perry, C.
Chant, D. C.
McLean, D.
Matigian, N.
Hayward, N. K.
McGrath, J. J.
Mackay-Sim, A.
Title of paper Functional and molecular alterations to cell cycle are seen in biopsied olfactory neuroepithelium from patients with schizophrenia and bipolar I disorder
Conference name XIIIth Biennial Winter Workshop on Schizophrenia Research
Conference location Davos, Switzerland
Conference dates 4-10 February, 2006
Proceedings title Schizophrenia Research : Abstracts of the XIIIth Biennial Winter Workshop on Schizophrenia Research   Check publisher's open access policy
Journal name Schizophrenia Research   Check publisher's open access policy
Place of Publication Amsterdam
Publisher Elsevier
Publication Year 2006
Sub-type Poster
DOI 10.1016/j.schres.2006.01.006
ISSN 0920-9964
Volume 81
Issue Supplement 1
Start page 198
End page 198
Total pages 1
Language eng
Formatted Abstract/Summary
Background:
Biopsy of olfactory neuroepithelium provides
an opportunity to examine neuronal tissue from individuals
with neuropsychiatric disorders. We previously demonstrated
that olfactory cultures from individuals with schizophrenia had
increased cell proliferation compared to cultures from healthy
controls. The aims of this study were to a) replicate this
observation in a new group of individuals with schizophrenia,
b) examine the specificity of these findings by including
individuals with bipolar I disorder and c) explore gene expression
differences that may underlie cell cycle differences in
these diseases.

Methods:

Olfactory mucosa biopsies were collected under
local anesthesia. Half the tissue was cultured in serum-free medium
and the other half underwent RNA extraction and microarray
analysis. We assessed attachment, mitosis, and cell death by
counting total cells, mitotic figures, and apoptotic/necrotic cells.
RNA samples underwent antisense amplification prior to microarray
hybridization.

Results:

Compared to controls, there was significantly more
mitosis in schizophrenia cultures (pb0.05) and significantly more
cell death in the bipolar I disorder cultures (pb0.005). In the
schizophrenia group (n=10), 0.65% of cells were in mitosis
compared to 0.25% and 0.29% for bipolar disorder (n=8) and
control (n=9) respectively. In the bipolar I disorder group, 6.12%
of cells were undergoing cell death compared to 2.90% and 1.8%
for the schizophrenia and control groups respectively. Microarray
data showed alterations to the cell cycle and phosphatidylinositol
signalling pathways in schizophrenia and bipolar I disorder
respectively.

Conclusions:

These distinct differences in cell proliferation,
cell death, and patterns of altered gene expression indicate
differential neurobiological correlates of schizophrenia versus
bipolar disorder. Cell cultures and gene expression based on
olfactory neuroepithelium provides an informative model for
studying key features of psychiatric diseases.
Subjects CX
110319 Psychiatry (incl. Psychotherapy)
111714 Mental Health
Keyword Psychiatry
Q-Index Code CX
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Created: Mon, 13 Aug 2007, 16:00:46 EST