A novel model to assess lamellar signaling relevant to preferential weight bearing in the horse

Gardner, A. K., van Eps, A. W., Watts, M. R., Burns, T. A. and Belknap, J. K. (2017) A novel model to assess lamellar signaling relevant to preferential weight bearing in the horse. Veterinary Journal, 221 62-67. doi:10.1016/j.tvjl.2017.02.005


Author Gardner, A. K.
van Eps, A. W.
Watts, M. R.
Burns, T. A.
Belknap, J. K.
Title A novel model to assess lamellar signaling relevant to preferential weight bearing in the horse
Journal name Veterinary Journal   Check publisher's open access policy
ISSN 1532-2971
1090-0233
Publication date 2017-03-01
Sub-type Article (original research)
DOI 10.1016/j.tvjl.2017.02.005
Open Access Status Not yet assessed
Volume 221
Start page 62
End page 67
Total pages 6
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2018
Language eng
Formatted abstract
Supporting limb laminitis (SLL) is a devastating sequela to severe unilateral lameness in equine patients. The manifestation of SLL, which usually only affects one limb, is unpredictable and the etiology is unknown. A novel, non-painful preferential weight bearing model designed to mimic the effects of severe unilateral forelimb lameness was developed to assess lamellar signaling events in the supporting limb (SL). A custom v-shaped insert was attached to the shoe of one forelimb to prevent normal weight bearing and redistribute weight onto the SL. Testing of the insert using a custom scale platform built into the floor of stocks confirmed increased distribution of weight on the SL compared with the unloaded forelimb (UL) and the contralateral (CH) and ipsilateral (IH) hind limbs in six Standardbred horses. In a second part of the study, eight healthy Standardbred horses were fitted with the insert and tied with consistent monitoring and free access to hay and water for 48 h, after which the lamellae were harvested. Real-time qPCR was performed to assess lamellar mRNA concentrations of inflammatory genes and immunoblotting and immunofluorescence were performed to assess lamellar protein concentration and cellular localization of hypoxia-related proteins, respectively. Lamellar mRNA concentrations of inflammatory signaling proteins did not differ between SL and either CH or IH samples. HIF-1α concentrations were greater (P < 0.05) in the SL compared to the CH. This work establishes an experimental model to study preferential weight bearing and initial results suggest that lamellar hypoxia may occur in the SL.
Keyword Cell signaling
HIF-1α
Hypoxia
Preferential weight bearing
Supporting limb laminitis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Veterinary Science Publications
 
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