Relationship between salivary/pancreatic amylase and body mass index: a systems biology approach

Bonnefond, Amélie, Yengo, Loïc, Dechaume, Aurélie, Canouil, Mickaël, Castelain, Maxime, Roger, Estelle, Allegaert, Frédéric, Caiazzo, Robert, Raverdy, Violeta, Pigeyre, Marie, Arredouani, Abdelilah, Borys, Jean-Michel, Levy-Marchal, Claire, Weill, Jacques, Roussel, Ronan, Balkau, Beverley, Marre, Michel, Pattou, François, Brousseau, Thierry and Froguel, Philippe (2017) Relationship between salivary/pancreatic amylase and body mass index: a systems biology approach. BMC Medicine, 15 1: . doi:10.1186/s12916-017-0784-x


Author Bonnefond, Amélie
Yengo, Loïc
Dechaume, Aurélie
Canouil, Mickaël
Castelain, Maxime
Roger, Estelle
Allegaert, Frédéric
Caiazzo, Robert
Raverdy, Violeta
Pigeyre, Marie
Arredouani, Abdelilah
Borys, Jean-Michel
Levy-Marchal, Claire
Weill, Jacques
Roussel, Ronan
Balkau, Beverley
Marre, Michel
Pattou, François
Brousseau, Thierry
Froguel, Philippe
Title Relationship between salivary/pancreatic amylase and body mass index: a systems biology approach
Journal name BMC Medicine   Check publisher's open access policy
ISSN 1741-7015
Publication date 2017-02-23
Sub-type Article (original research)
DOI 10.1186/s12916-017-0784-x
Open Access Status DOI
Volume 15
Issue 1
Total pages 10
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2018
Language eng
Formatted abstract
Background: Salivary (AMY1) and pancreatic (AMY2) amylases hydrolyze starch. Copy number of AMY1A (encoding AMY1) was reported to be higher in populations with a high-starch diet and reduced in obese people. These results based on quantitative PCR have been challenged recently. We aimed to re-assess the relationship between amylase and adiposity using a systems biology approach.

Methods: We assessed the association between plasma enzymatic activity of AMY1 or AMY2, and several metabolic traits in almost 4000 French individuals from D.E.S.I.R. longitudinal study. The effect of the number of copies of AMY1A (encoding AMY1) or AMY2A (encoding AMY2) measured through droplet digital PCR was then analyzed on the same parameters in the same study. A Mendelian randomization analysis was also performed. We subsequently assessed the association between AMY1A copy number and obesity risk in two case-control studies (5000 samples in total). Finally, we assessed the association between body mass index (BMI)-related plasma metabolites and AMY1 or AMY2 activity.

Results: We evidenced strong associations between AMY1 or AMY2 activity and lower BMI. However, we found a modest contribution of AMY1A copy number to lower BMI. Mendelian randomization identified a causal negative effect of BMI on AMY1 and AMY2 activities. Yet, we also found a significant negative contribution of AMY1 activity at baseline to the change in BMI during the 9-year follow-up, and a significant contribution of AMY1A copy number to lower obesity risk in children, suggesting a bidirectional relationship between AMY1 activity and adiposity. Metabonomics identified a BMI-independent association between AMY1 activity and lactate, a product of complex carbohydrate fermentation.

Conclusions: These findings provide new insights into the involvement of amylase in adiposity and starch metabolism.
Keyword AMY1A/AMY2A
Body mass index
Copy number variant
Mendelian randomization
Metabonomics
Obesity
Salivary/Pancreatic amylase
Starch
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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