Isolation of wheat endosperm cell walls: effects of non-endosperm flour components on structural analyses

Gartaula, Ghanendra, Dhital, Sushil, Pleming, Denise and Gidley, Michael J. (2017) Isolation of wheat endosperm cell walls: effects of non-endosperm flour components on structural analyses. Journal of Cereal Science, 74 165-173. doi:10.1016/j.jcs.2017.02.004


Author Gartaula, Ghanendra
Dhital, Sushil
Pleming, Denise
Gidley, Michael J.
Title Isolation of wheat endosperm cell walls: effects of non-endosperm flour components on structural analyses
Journal name Journal of Cereal Science   Check publisher's open access policy
ISSN 1095-9963
0733-5210
Publication date 2017-03-01
Sub-type Article (original research)
DOI 10.1016/j.jcs.2017.02.004
Open Access Status Not yet assessed
Volume 74
Start page 165
End page 173
Total pages 9
Place of publication London, United Kingdom
Publisher Academic Press
Collection year 2018
Language eng
Abstract We report the isolation of a pure form of cell walls from wheat endosperm ‘popped’ out from the whole, enzyme deactivated and soaked grain, and compare them with cell walls isolated from milled flours of extraction rates from 45% to 75%, at physiological (37 °C) and elevated (70 °C) temperatures. Cell walls isolated from flours all contained non-endosperm walls whereas walls from popped endosperm were apparently pure. The monosaccharide composition of ‘popped’ cell walls was different to that of cell walls isolated from flour, particularly glucose and mannose contents (34 and 7% for ‘popped’ cf 29 and 3% for flour respectively) and arabinose to xylose ratios (0.45 for ‘popped’ cf 0.58 for flour). Total phenolic contents of popped endosperm cell walls were three to four times lower than for cell walls from flour. Elevated isolation temperature also had a solubilising effect, altering the cell wall composition. This study provides a novel method of isolating pure wheat endosperm cell walls, and demonstrates how contaminating (thick cell walled) non-endospermic material in milled flours can have a major influence on cell wall compositional analyses.
Keyword Aleurone
Cell walls
Endosperm
Wheat
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 14 Mar 2017, 00:24:17 EST by System User on behalf of Learning and Research Services (UQ Library)