Multi-centre assessment of nitroblue tetrazolium reactivity in human semen as a potential marker of oxidative stress

Gosalvez, Jaime, Coppola, Lamberto, Fernandez, Jose Luis, Lopez-Fernandez, Carmen, Gongora, Alfredo, Sanitso, Ricardo, Harrison, Keith, Agarwal, Ashok, Johnston, Stephen and Esteves, Sandro (2017) Multi-centre assessment of nitroblue tetrazolium reactivity in human semen as a potential marker of oxidative stress. Reproductive BioMedicine Online, . doi:10.1016/j.rbmo.2017.01.014


Author Gosalvez, Jaime
Coppola, Lamberto
Fernandez, Jose Luis
Lopez-Fernandez, Carmen
Gongora, Alfredo
Sanitso, Ricardo
Harrison, Keith
Agarwal, Ashok
Johnston, Stephen
Esteves, Sandro
Title Multi-centre assessment of nitroblue tetrazolium reactivity in human semen as a potential marker of oxidative stress
Journal name Reproductive BioMedicine Online   Check publisher's open access policy
ISSN 1472-6483
1472-6491
Publication date 2017-03-08
Year available 2017
Sub-type Article (original research)
DOI 10.1016/j.rbmo.2017.01.014
Open Access Status Not yet assessed
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Elsevier
Collection year 2018
Language eng
Formatted abstract
The nitroblue tetrazolium (NBT) reaction as a tracer of oxidative stress was examined in 707 ejaculates from seven clinics. Semen was initially surveyed by classifying the NBT reaction using a pre-established rank for the Oxisperm® test based on three colourimetric levels: L1, low (n = 141 [20%]); L2, medium (n = 538 [76%]) and L3, high (n = 28 [4%]). L3 was indicative of a high level of superoxide anions. Halosperm® chromatin dispersion assay was used to analyse samples of ejaculates 30 min after ejaculation; no difference was found in DNA fragmentation of L1 or L3; L3 category semen samples incubated for 24 h at 37oC showed a significantly faster rate (P < 0.001) of DNA damage than those in L1. The NBT reaction was further characterized in the ejaculates of 100 patients to determine the relative contribution of seminal plasma, spermatozoa, or both. Seminal plasma was the most significant fraction of •O2− localization, whereas sperm fractions generated detectable reactive oxygen species in only 32% of the ejaculates. Formazan precipitates were primarily associated with the sperm mid-piece and seminal leukocytes; however, not all spermatozoa stained positive to formazan and not all leukocytes presented with equivalent production of superoxide anions.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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Created: Wed, 08 Mar 2017, 00:50:22 EST by Associate Professor Stephen Johnston on behalf of School of Agriculture and Food Sciences