The effects of C5aR1 on leukocyte infiltration following pilocarpine-induced status epilepticus

Benson, Melissa J., Manzanero, Silvia and Borges, Karin (2017) The effects of C5aR1 on leukocyte infiltration following pilocarpine-induced status epilepticus. Epilepsia, . doi:10.1111/epi.13698


Author Benson, Melissa J.
Manzanero, Silvia
Borges, Karin
Title The effects of C5aR1 on leukocyte infiltration following pilocarpine-induced status epilepticus
Journal name Epilepsia   Check publisher's open access policy
ISSN 1528-1167
0013-9580
Publication date 2017-02-22
Sub-type Article (original research)
DOI 10.1111/epi.13698
Open Access Status Not yet assessed
Total pages 5
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Collection year 2018
Language eng
Formatted abstract
This study aimed to determine the role C5aR1 plays in mediating immune responses acutely after pilocarpine-induced status epilepticus (SE), specifically those of brain-infiltrating leukocytes. Three days following pilocarpine SE, we determined by flow cytometry the brain immune cell phenotypes and measured key proinflammatory and antiinflammatory cytokine expression by infiltrating leukocytes and microglia in C5aR1-deficient and wild-type mice. Absence of C5aR1 reduced by 47% the numbers of Ly6G+ neutrophils in the brains of No-SE mice and decreased neutrophil entry after SE to levels found in wild-type brains that did not undergo SE (No-SE). Moreover, C5aR1-deficient mice showed increased interleukin (IL)-4 expression in infiltrating leukocytes, but not in microglia. Increases in IL-4 expression in infiltrating leukocytes coupled with decreased neutrophil invasion in C5aR1-deficient mice after SE is likely to contribute to the reduced neuronal loss previously found in these mice compared to their wild-type littermates. Although other SE models need to be investigated to substantiate our findings, this study provides further evidence that C5aR1 is an inflammatory mediator and may play a role in epileptogenesis.
Keyword C5a receptor 1
Complement
Interleukin-4
Neuroinflammation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 07 Mar 2017, 00:20:21 EST by System User on behalf of Learning and Research Services (UQ Library)