A biosensor of local kinesin activity reveals roles of PKC and EB1 in KIF17 activation

Espenel, Cedric, Acharya, Bipul R. and Kreitzer, Geri (2013) A biosensor of local kinesin activity reveals roles of PKC and EB1 in KIF17 activation. Journal of Cell Biology, 203 3: 445-455. doi:10.1083/jcb.201305023

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Author Espenel, Cedric
Acharya, Bipul R.
Kreitzer, Geri
Title A biosensor of local kinesin activity reveals roles of PKC and EB1 in KIF17 activation
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 2013-11-04
Sub-type Article (original research)
DOI 10.1083/jcb.201305023
Open Access Status File (Publisher version)
Volume 203
Issue 3
Start page 445
End page 455
Total pages 11
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract We showed previously that the kinesin-2 motor KIF17 regulates microtubule (MT) dynamics and organization to promote epithelial differentiation. How KIF17 activity is regulated during this process remains unclear. Several kinesins, including KIF17, adopt compact and extended conformations that reflect autoinhibited and active states, respectively. We designed biosensors of KIF17 to monitor its activity directly in single cells using fluorescence lifetime imaging to detect Förster resonance energy transfer. Lifetime data are mapped on a phasor plot, allowing us to resolve populations of active and inactive motors in individual cells. Using this biosensor, we demonstrate that PKC contributes to the activation of KIF17 and that this is required for KIF17 to stabilize MTs in epithelia. Furthermore, we show that EB1 recruits KIF17 to dynamic MTs, enabling its accumulation at MT ends and thus promoting MT stabilization at discrete cellular domains.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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