Deciphering the role of Atg5 in nucleotide dependent interaction of Rab33B with the dimeric complex, Atg5-Atg16L1

Chandra, Mintu, Saran, Runjhun and Datta, Sunando (2016) Deciphering the role of Atg5 in nucleotide dependent interaction of Rab33B with the dimeric complex, Atg5-Atg16L1. Biochemical and Biophysical Research Communications, 473 1: 8-16. doi:10.1016/j.bbrc.2016.03.043


Author Chandra, Mintu
Saran, Runjhun
Datta, Sunando
Title Deciphering the role of Atg5 in nucleotide dependent interaction of Rab33B with the dimeric complex, Atg5-Atg16L1
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
0006-291X
Publication date 2016-04-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2016.03.043
Open Access Status Not yet assessed
Volume 473
Issue 1
Start page 8
End page 16
Total pages 9
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Autophagy is a lysosomal degradation pathway that degrades cytosolic constituents, including whole organelles and intracellular pathogens. Previous studies on various autophagy related genes revealed the importance of the Atg12–Atg5–Atg16 complex in autophagy. Atg16L1 is an effector of Golgi-resident Rab33B and the molecular mechanism of the interaction of Rab33B with either Atg16L1 or in complex with Atg5 is still elusive. In the current study, using the pull down and calorimetric approaches, we have dissected the molecular insights into the interaction of Rab33B with different regions of mouse Atg16L1 as well as with the dimeric complex, Atg5-mAtg16L1. Our in vitro observation suggests that Atg5 is pre-requisite for the augmented nucleotide dependent interaction of Rab33B with the dimeric complex, Atg5-Atg16L1. Moreover, the results reported here suggest that Arg-24 of Atg16L1 is crucial for its interaction with Atg5 which will have further implication in the binding of the dimeric complex to Rab33B.
Keyword Autophagy
GST-pull down
Rab GTPase
Size exclusion chromatography
Thermodynamics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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