Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis

Sotillo, Javier, Ferreira, Ivana, Potriquet, Jeremy, Laha, Thewarach, Navarro, Severine, Loukas, Alex and Mulvenna, Jason (2017) Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis. Scientific Reports, 7 . doi:10.1038/srep41883


Author Sotillo, Javier
Ferreira, Ivana
Potriquet, Jeremy
Laha, Thewarach
Navarro, Severine
Loukas, Alex
Mulvenna, Jason
Title Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis
Formatted title
Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2017-02-13
Sub-type Article (original research)
DOI 10.1038/srep41883
Open Access Status DOI
Volume 7
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2018
Language eng
Formatted abstract
Different reports have highlighted the potential use of helminths and their secretions in the treatment of inflammatory bowel disease (IBD) conditions; however, no reports have investigated their effects at a proteome level. Herein, we characterise the protein expression changes that occur in lamina propria (LP) and the intestinal epithelial cells (IEC) of mice with dextran sulfate sodium (DSS)-induced colitis treated with Ancylostoma caninum excretory/secretory (ES) products using a quantitative proteomic approach. We have shown how parasite products can significantly alter the expression of proteins involved in immune responses, cell death and with an antioxidant activity. Interestingly, significant changes in the expression levels of different mucins were observed in this study. MUC13, a mucin implicated in gastrointestinal homeostasis, was upregulated in the LP of mice with DSS-induced colitis treated with ES, while MUC2, a major component of mucus, was upregulated in the IEC. In addition, A. caninum proteins have an important effect on proteins with antioxidant functions and proteins involved in intestinal homeostasis and tissue integrity and regeneration. Understanding how parasites can ameliorate IBD pathogenesis can help us design novel treatments for autoimmune diseases.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biomedical Sciences Publications
 
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