Maturing reticulocytes internalize plasma membrane in glycophorin A-containing vesicles that fuse with autophagosomes before exocytosis

Griffiths, Rebecca E., Kupzig, Sabine, Cogan, Nicola, Mankelow, Tosti J., Betin, Virginie M. S., Trakarnsanga, Kongtana, Massey, Edwin J. , Lane, Jon D., Parsons, Stephen F. and Anstee, David J. (2012) Maturing reticulocytes internalize plasma membrane in glycophorin A-containing vesicles that fuse with autophagosomes before exocytosis. Blood, 119 26: 6296-6306. doi:10.1182/blood-2011-09-376475


Author Griffiths, Rebecca E.
Kupzig, Sabine
Cogan, Nicola
Mankelow, Tosti J.
Betin, Virginie M. S.
Trakarnsanga, Kongtana
Massey, Edwin J.
Lane, Jon D.
Parsons, Stephen F.
Anstee, David J.
Title Maturing reticulocytes internalize plasma membrane in glycophorin A-containing vesicles that fuse with autophagosomes before exocytosis
Journal name Blood   Check publisher's open access policy
ISSN 0006-4971
1528-0020
Publication date 2012-06-28
Sub-type Article (original research)
DOI 10.1182/blood-2011-09-376475
Open Access Status Not yet assessed
Volume 119
Issue 26
Start page 6296
End page 6306
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society of Hematology
Language eng
Formatted abstract
The erythrocyte is one of the best characterized human cells. However, studies of the process whereby human reticulocytes mature to erythrocytes have been hampered by the difficulty of obtaining sufficient numbers of cells for analysis. In the present study, we describe an in vitro culture system producing milliliter quantities of functional mature human adult reticulocytes from peripheral blood CD34+ cells. We show that the final stage of reticulocyte maturation occurs by a previously undescribed mechanism in which large glycophorin A–containing vesicles forming at the cytosolic face of the plasma membrane are internalized and fuse with autophagosomes before expulsion of the autophagosomal contents by exocytosis. Early reticulocyte maturation is characterized by the selective elimination of unwanted plasma membrane proteins (CD71, CD98, and β1 integrin) through the endosome-exosome pathway. In contrast, late maturation is characterized by the generation of large glycophorin A–decorated vesicles of autophagic origin.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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