Molecular bacterial load assay, a culture-free biomarker for rapid and accurate quantification of sputum Mycobacterium tuberculosis bacillary load during treatment

Honeyborne, Isobella, McHugh, Timothy D., Phillips, Patrick P.J., Bannoo, Selina, Bateson, Anna, Carroll, Nora, Perrin, Felicity M., Ronacher, Katharina, Wright, Laura, Van Helden, Paul D., Walzl, Gerhard and Gillespie, Stephen H. (2011) Molecular bacterial load assay, a culture-free biomarker for rapid and accurate quantification of sputum Mycobacterium tuberculosis bacillary load during treatment. Journal of Clinical Microbiology, 49 11: 3905-3911. doi:10.1128/JCM.00547-11

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Author Honeyborne, Isobella
McHugh, Timothy D.
Phillips, Patrick P.J.
Bannoo, Selina
Bateson, Anna
Carroll, Nora
Perrin, Felicity M.
Ronacher, Katharina
Wright, Laura
Van Helden, Paul D.
Walzl, Gerhard
Gillespie, Stephen H.
Title Molecular bacterial load assay, a culture-free biomarker for rapid and accurate quantification of sputum Mycobacterium tuberculosis bacillary load during treatment
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 2011-11-01
Sub-type Article (original research)
DOI 10.1128/JCM.00547-11
Open Access Status File (Publisher version)
Volume 49
Issue 11
Start page 3905
End page 3911
Total pages 7
Place of publication United States
Publisher American Society for Microbiology
Language eng
Abstract A molecular assay to quantify Mycobacterium tuberculosis is described. In vitro, 98% (n = 96) of sputum samples with a known number of bacilli (107 to 102 bacilli) could be enumerated within 0.5 log10. In comparison to culture, the molecular bacterial load (MBL) assay is unaffected by other microorganisms present in the sample, results are obtained more quickly (within 24 h) and are seldom inhibited (0.7% samples), and the MBL assay critically shows the same biphasic decline as observed longitudinally during treatment. As a biomarker of treatment response, the MBL assay responds rapidly, with a mean decline in bacterial load for 111 subjects of 0.99 log10 (95% confidence interval [95% CI], 0.81 to 1.17) after 3 days of chemotherapy. There was a significant association between the rate of bacterial decline during the same 3 days and bacilli ml−1 sputum at day 0 (linear regression, P = 0.0003) and a 3.62 increased odds ratio of relapse for every 1 log10 increase in pretreatment bacterial load (95% CI, 1.53 to 8.59).
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
 
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