A proteome analysis of conditioned media from human neonatal fibroblasts used in the maintenance of human embryonic stem cells

Prowse, Andrew B. J., McQuade, Leon R., Bryant, Katherin J., Van Dyk, Derek D., Tuch, BBernard E. and Gray, Peter P. (2005). A proteome analysis of conditioned media from human neonatal fibroblasts used in the maintenance of human embryonic stem cells. In: Special Issue: Proceedings of the 3rd International Proteomics Conference held conjointly with the 1st Taiwan Proteomics Conference and the 2nd AOHUPO Congress. 3rd International Proteomics Conference/1st Taiwan Proteomics Conference/2nd AOHUPO Congress, Taipei, Taiwan, (978-989). 14-17 May 2004. doi:10.1002/pmic.200401087


Author Prowse, Andrew B. J.
McQuade, Leon R.
Bryant, Katherin J.
Van Dyk, Derek D.
Tuch, BBernard E.
Gray, Peter P.
Title of paper A proteome analysis of conditioned media from human neonatal fibroblasts used in the maintenance of human embryonic stem cells
Conference name 3rd International Proteomics Conference/1st Taiwan Proteomics Conference/2nd AOHUPO Congress
Conference location Taipei, Taiwan
Conference dates 14-17 May 2004
Proceedings title Special Issue: Proceedings of the 3rd International Proteomics Conference held conjointly with the 1st Taiwan Proteomics Conference and the 2nd AOHUPO Congress   Check publisher's open access policy
Journal name Proteomics   Check publisher's open access policy
Place of Publication Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Publication Year 2005
Sub-type Fully published paper
DOI 10.1002/pmic.200401087
ISSN 1615-9853
Volume 5
Issue 4
Start page 978
End page 989
Total pages 12
Language eng
Abstract/Summary The pathways involved in the maintenance of human embryonic stem (hES) cells remain largely unknown, although some signaling pathways have been identified in mouse embryonic stem (mES) cells. Fibroblast feeder layers are used to maintain the undifferentiated growth of hES cells and an examination of the conditioned media (CM) of human neonatal fibroblasts (HNFs) could provide insights into the maintenance of hES cells. The neonatal foreskin fibroblast line (HNF02) used in this study was shown to have a normal 2n = 46, XY chromosomal complement and to support the undifferentiated growth of the Embryonic Stem Cell International Pte. Ltd.-hES3 cell line. The CM of HNF02 was examined using two-dimensional liquid chromatography-tandem mass spectrometry (2-D LCMS) and two-dimensional electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization-time of flight tandem mass spectrometry (2-DE/MALDI). A total of 102 proteins were identified, 19 by 2-DE/MALDI, 53 by 2-D LCMS and 30 by both techniques. These proteins were classified into 15 functional groups. Proteins identified in the extracellular matrix and differentiation and growth factor functional categories were considered most likely to be involved in the maintenance of hES cell growth, differentiation and pluripotency as these groups contained proteins involved in a variety of events including cell adhesion, cell proliferation and inhibition of cell proliferation, Writ signaling and inhibition of bone morphogenetic proteins.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Conditioned Media
Human Embryonic Stem Cells
Human Neonatal Fibroblasts
Pluripotency
Proteome Analysis
Growth-factor-beta
Prolonged Undifferentiated Growth
Microvascular Endothelial-cells
Binding Protein-3
Transforming Growth-factor-beta-1
Hematopoietic-cells
Mass-spectrometry
Feeder Layers
Sparc
Decorin
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

 
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Created: Mon, 13 Aug 2007, 15:05:16 EST