Chitooligosaccharides attenuate UVB-induced damages in human dermal fibroblasts

Ahn, Byul-Nim, Kim, Jung-Ae, Himaya, S. W. A., Bak, Soon-Sun, Kong, Chang-Suk and Kim, Se-Kwon (2012) Chitooligosaccharides attenuate UVB-induced damages in human dermal fibroblasts. Naunyn-Schmiedeberg's Archives of Pharmacology, 385 1: 95-102. doi:10.1007/s00210-011-0684-0


Author Ahn, Byul-Nim
Kim, Jung-Ae
Himaya, S. W. A.
Bak, Soon-Sun
Kong, Chang-Suk
Kim, Se-Kwon
Title Chitooligosaccharides attenuate UVB-induced damages in human dermal fibroblasts
Journal name Naunyn-Schmiedeberg's Archives of Pharmacology   Check publisher's open access policy
ISSN 0028-1298
1432-1912
Publication date 2012-01-01
Sub-type Article (original research)
DOI 10.1007/s00210-011-0684-0
Open Access Status Not yet assessed
Volume 385
Issue 1
Start page 95
End page 102
Total pages 8
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract As a continuation of our research on chitooligosaccharides (COS), this study focused on the protective effect of COS of various molecular weights (1–3, 3–5, and 5–10 kDa) on cellular damage caused by ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblast cells. The results show that the protective effect of COS on UVB-stressed human fibroblasts was dependent on molecular weight. COS suppressed UVB irradiation-induced reactive oxygen species generation and DNA damage, accompanied by the downregulation of matrix metalloproteinase (MMP)-1 and MMP-13. In a comparative analysis, COS (3–5 kDa) exhibited the most potent protective effect on UVB-stressed fibroblasts. The presence of COS (3–5 kDa) attenuated UVB-induced collagenolytic MMP production and collagen degradation. The photoprotective activity of COS (3–5 kDa) was confirmed by transcriptional phosphorylation of mitogen-activated protein kinase-responsive signaling pathways.
Keyword Ultraviolet-B
Human dermal fibroblasts
Chitooligosaccharides
MMP
Photoaging
MAPK
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes As a continuation of our research on chitooligosaccharides (COS), this study focused on the protective effect of COS of various molecular weights (1–3, 3–5, and 5–10 kDa) on cellular damage caused by ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblast cells. The results show that the protective effect of COS on UVB-stressed human fibroblasts was dependent on molecular weight. COS suppressed UVB irradiation-induced reactive oxygen species generation and DNA damage, accompanied by the downregulation of matrix metalloproteinase (MMP)-1 and MMP-13. In a comparative analysis, COS (3–5 kDa) exhibited the most potent protective effect on UVB-stressed fibroblasts. The presence of COS (3–5 kDa) attenuated UVB-induced collagenolytic MMP production and collagen degradation. The photoprotective activity of COS (3–5 kDa) was confirmed by transcriptional phosphorylation of mitogen-activated protein kinase-responsive signaling pathways.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 23 Jan 2017, 10:48:29 EST by Himaya Siddhihalu Wickrama Hewage on behalf of Institute for Molecular Bioscience