Identification of IncA/C plasmid replication and maintenance genes and development of a plasmid multi-locus sequence-typing scheme

Hancock, Steven J., Phan, Minh-Duy, Peters, Kate M., Forde, Brian M., Chong, Teik Min, Yin, Wai-Fong, Chan, Kok-Gan, Paterson, David L., Walsh, Timothy R., Beatson, Scott A. and Schembri, Mark A. (2016) Identification of IncA/C plasmid replication and maintenance genes and development of a plasmid multi-locus sequence-typing scheme. Antimicrobial Agents and Chemotherapy, 61 2: . doi:10.1128/AAC.01740-16

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Author Hancock, Steven J.
Phan, Minh-Duy
Peters, Kate M.
Forde, Brian M.
Chong, Teik Min
Yin, Wai-Fong
Chan, Kok-Gan
Paterson, David L.
Walsh, Timothy R.
Beatson, Scott A.
Schembri, Mark A.
Title Identification of IncA/C plasmid replication and maintenance genes and development of a plasmid multi-locus sequence-typing scheme
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 1098-6596
Publication date 2016-11-21
Year available 2016
Sub-type Article (original research)
DOI 10.1128/AAC.01740-16
Open Access Status File (Author Post-print)
Volume 61
Issue 2
Total pages 1
Place of publication Washington, DC United States
Publisher American Society for Microbiology
Collection year 2017
Language eng
Formatted abstract
Plasmids of incompatibility group IncA/C are becoming increasingly prevalent within pathogenic Enterobacteriaceae. They are associated with the dissemination of multiple clinically relevant resistance genes, including blaCMY and blaNDM. Current typing methods for IncA/C plasmids offer limited resolution. In this study, we present the complete sequence of a blaNDM-1-positive IncA/C plasmid, pMS6198A, isolated from a multi-drug resistant uropathogenic Escherichia coli strain. Hyper-saturated transposon mutagenesis, coupled with transposon directed insertion site sequencing (TraDIS) was employed to identify conserved genetic elements required for replication and maintenance of pMS6198A. Our analysis of TraDIS data identified roles for the replicon, including repA, a toxin-antitoxin system, two putative partitioning genes parAB and a putative gene, 053. Construction of mini-IncA/C plasmids and examination of their stability within E. coli confirmed that the region encompassing 053 contributes to the stable maintenance of IncA/C plasmids. Subsequently, the four major maintenance genes (repA, parAB, 053) were used to construct a new plasmid multi-locus sequence typing (PMLST) scheme for IncA/C plasmids. Application of this scheme to a database of 82 IncA/C plasmids identified eleven unique sequence types (STs), with two dominant STs. The majority of blaNDM positive plasmids examined (15/17, 88%) fall into ST1, suggesting acquisition and subsequent expansion of this blaNDM-containing plasmid lineage. The IncA/C PMLST scheme represents a standardized tool to identify, track and analyse the dissemination of important IncA/C plasmid lineages, particularly in the context of epidemiological studies.
Keyword Uropathogenic E. coli
IncA/C plasmid
Functional genomics
New-Delhi 19 metallo-beta-lactamase
Plasmid multi locus sequence typing
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Fri, 13 Jan 2017, 21:31:00 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences