Pulse and surge profiles of luteinizing hormone secretion in the mouse

Czieselsky, Katja, Prescott, Mel, Porteous, Robert, Campos, Pauline, Clarkson, Jenny, Steyn, Frederik J., Campbell, Rebecca E. and Herbison, Allan E. (2016) Pulse and surge profiles of luteinizing hormone secretion in the mouse. Endocrinology, 157 12: 4794-4802. doi:10.1210/en.2016-1351


Author Czieselsky, Katja
Prescott, Mel
Porteous, Robert
Campos, Pauline
Clarkson, Jenny
Steyn, Frederik J.
Campbell, Rebecca E.
Herbison, Allan E.
Title Pulse and surge profiles of luteinizing hormone secretion in the mouse
Journal name Endocrinology   Check publisher's open access policy
ISSN 1945-7170
0013-7227
Publication date 2016-12-01
Sub-type Article (original research)
DOI 10.1210/en.2016-1351
Open Access Status Not yet assessed
Volume 157
Issue 12
Start page 4794
End page 4802
Total pages 9
Place of publication Cary, NC, United States
Publisher Oxford University Press
Collection year 2017
Language eng
Abstract Using a new tail-tip bleeding procedure and a sensitive ELISA, we describe here the patterns of LH secretion throughout the mouse estrous cycle; in ovariectomized mice; in ovariectomized, estradiol-treated mice that model estrogen-negative and-positive feedback; and in transgenic GNR23 mice that exhibit allele-dependent reductions in GnRH neuron number. Pulsatile LH secretion was evident at all stages of the estrous cycle, with LH pulse frequency being approximately one pulse per hour in metestrous, diestrous, and proestrous mice but much less frequent at estrus (less than one pulse per 4 h). Ovariectomy resulted in substantial increases in basal and pulsatile LH secretion with pulses occurring approximately every 21 minutes. Chronic treatment with negative-feedback, estradiol-filled capsules returned LH pulse frequency to intact follicular phase levels, although pulse amplitude remained elevated. On the afternoon of proestrus, the LH surge was found to begin in a highly variable manner over a 4-hour range, lasting for more than 3 hours. In contrast, ovariectomized, estradiol-treated, positive-feedback mice exhibited a relatively uniform surge onset at approximately 0.5 hour prior to lights out. Gonadectomized wild-type and heterozygous GNR23 (β200 GnRH neurons) male mice exhibited an LH pulse every 60 minutes. Homozygous GNR23 mice (β80 GnRH neurons) had very low basal LH concentrations but continued to exhibit small amplitude LH pulses every 90 minutes. These studies provide the first characterization in mice of pulse and surge modes of LH secretion across the estrous cycle and demonstrate that very few GnRH neurons are required for pulsatile LH secretion.
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Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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