Beyond antibodies: development of a novel protein scaffold based on human chaperonin 10

Alsultan, Abdulkarim M., Chin, David Y., Howard, Christopher B., de Bakker, Christopher J., Jones, Martina L. and Mahler, Stephen M. (2016) Beyond antibodies: development of a novel protein scaffold based on human chaperonin 10. Scientific Reports, 5 37348: . doi:10.1038/srep37348


Author Alsultan, Abdulkarim M.
Chin, David Y.
Howard, Christopher B.
de Bakker, Christopher J.
Jones, Martina L.
Mahler, Stephen M.
Title Beyond antibodies: development of a novel protein scaffold based on human chaperonin 10
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-11-22
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep37348
Open Access Status DOI
Volume 5
Issue 37348
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Formatted abstract
Human Chaperonin 10 (hCpn10) was utilised as a novel scaffold for presenting peptides of therapeutic and diagnostic significance. Molecular dynamic simulations and protein sizing analyses identified a peptide linker (P1) optimal for the formation of the quarternary hCpn10 heptamer structure. hCpn10 scaffold displaying peptides targeting Factor VIIa (CE76-P1) and CD44 (CP7) were expressed in E. coli. Functional studies of CE76-P1 indicated nanomolar affinity for Factor VIIa (3 nM) similar to the E-76 peptide (6 nM), with undetectable binding to Factor X. CE76-P1 was a potent inhibitor of FX activity (via inhibition of Factor VIIa) and prolonged clot formation 4 times longer than achieved by E-76 peptide as determined by prothrombin time (PT) assays. This improvement in clotting function by CE76-P1, highlights the advantages of a heptamer-based scaffold for improving avidity by multiple peptide presentation. In another example of hCPn10 utility as a scaffold, CP7 bound to native CD44 overexpressed on cancer cells and bound rCD44 with high affinity (KD 9.6 nM). The ability to present various peptides through substitution of the hCpn10 mobile loop demonstrates its utility as a novel protein scaffold.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Tue, 06 Dec 2016, 10:49:56 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology