Whole genome sequencing reveals potential new targets for improving nitrogen uptake and utilization in Sorghum bicolor

Massel, Karen, Campbell, Bradley C., Mace, Emma S., Tai, Shuaishuai, Tao, Yongfu, Worland, Belinda G., Jordan, David R., Botella, Jose R. and Godwin, Ian D. (2016) Whole genome sequencing reveals potential new targets for improving nitrogen uptake and utilization in Sorghum bicolor. Frontiers in Plant Science, 7 OCTOBER2016: . doi:10.3389/fpls.2016.01544


Author Massel, Karen
Campbell, Bradley C.
Mace, Emma S.
Tai, Shuaishuai
Tao, Yongfu
Worland, Belinda G.
Jordan, David R.
Botella, Jose R.
Godwin, Ian D.
Title Whole genome sequencing reveals potential new targets for improving nitrogen uptake and utilization in Sorghum bicolor
Formatted title
Whole genome sequencing reveals potential new targets for improving nitrogen uptake and utilization in Sorghum bicolor
Journal name Frontiers in Plant Science   Check publisher's open access policy
ISSN 1664-462X
Publication date 2016-10-25
Sub-type Article (original research)
DOI 10.3389/fpls.2016.01544
Open Access Status DOI
Volume 7
Issue OCTOBER2016
Total pages 15
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2017
Language eng
Formatted abstract
Nitrogen (N) fertilizers are a major agricultural input where more than 100 million tons are supplied annually. Cereals are particularly inefficient at soil N uptake, where the unrecovered nitrogen causes serious environmental damage. Sorghum bicolor (sorghum) is an important cereal crop, particularly in resource-poor semi-arid regions, and is known to have a high NUE in comparison to other major cereals under limited N conditions. This study provides the first assessment of genetic diversity and signatures of selection across 230 fully sequenced genes putatively involved in the uptake and utilization of N from a diverse panel of sorghum lines. This comprehensive analysis reveals an overall reduction in diversity as a result of domestication and a total of 128 genes displaying signatures of purifying selection, thereby revealing possible gene targets to improve NUE in sorghum and cereals alike. A number of key genes appear to have been involved in selective sweeps, reducing their sequence diversity. The ammonium transporter (AMT) genes generally had low allelic diversity, whereas a substantial number of nitrate/peptide transporter 1 (NRT1/PTR) genes had higher nucleotide diversity in domesticated germplasm. Interestingly, members of the distinct race Guinea margaritiferum contained a number of unique alleles, and along with the wild sorghum species, represent a rich resource of new variation for plant improvement of NUE in sorghum.
Keyword Nitrogen uptake and utilization
Nitrogen use efficiency (NUE)
Sorghum (Sorghum bicolor)
Domestication
Selection
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
 
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