Persistence of recipient lymphocytes in NOD mice after irradiation and bone marrow transplantation

Steptoe, Raymond J., Stankovic, Sanda, Lopaticki, Sash, Jones, Lynelle K., Harrison, Leonard C. and Morahan, Grant (2004) Persistence of recipient lymphocytes in NOD mice after irradiation and bone marrow transplantation. Journal of Autoimmunity, 22 2: 131-138. doi:10.1016/j.jaut.2003.12.003

Author Steptoe, Raymond J.
Stankovic, Sanda
Lopaticki, Sash
Jones, Lynelle K.
Harrison, Leonard C.
Morahan, Grant
Title Persistence of recipient lymphocytes in NOD mice after irradiation and bone marrow transplantation
Journal name Journal of Autoimmunity   Check publisher's open access policy
ISSN 0896-8411
Publication date 2004
Sub-type Article (original research)
DOI 10.1016/j.jaut.2003.12.003
Volume 22
Issue 2
Start page 131
End page 138
Total pages 8
Place of publication London
Publisher Academic Press Ltd Elsevier Science Ltd
Language eng
Subject 1107 Immunology
Abstract The non-obese diabetic (NOD) mouse is a unique and invaluable model of autoimmune disease, in particular type I diabetes. Bone marrow transplantation as a therapy for type I diabetes has been explored in NOD mice. NOD mice require higher doses of conditioning irradiation for successful allogeneic bone marrow transplantation, suggesting that NOD hematopoietic cells are radioresistant compared to those of other mouse strains. However, studies of hematopoietic reconstitution in NOD mice are hampered by the lack of mice bearing a suitable cell-surface marker that would allow transferred cells or their progeny to be distinguished. In order to monitor hematopoietic reconstitution in NOD mice we generated congenic NOD mice that carry the alternative allelic form of the pan-leukocyte alloantigen CD45. Following irradiation and congenic bone marrow transplantation, we found that the myeloid lineage was rapidly reconstituted by cells of donor origin but substantial numbers of recipient T lymphocytes persisted even after supra-lethal irradiation. This indicates that radiation resistance in the NOD hematopoietic compartment is a property primarily of mature T lymphocytes. (C) 2004 Elsevier Ltd. All rights reserved.
Keyword Immunology
Radiation Resistance
Hematopoietic Stem-cells
Apoptosis Resistance
Adoptive Transfer
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Diamantina Institute Publications
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Created: Mon, 13 Aug 2007, 14:11:37 EST