Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB

Wang, Tong, Martin, Sally, Nguyen, Tam H., Harper, Callista B., Gormal, Rachel S., Martinez-Marmol, Ramon, Karunanithi, Shanker, Coulson, Elizabeth J., Glass, Nick R., Cooper-White, Justin J., Van Swinderen, Bruno and Meunier, Frederic A. (2016) Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB. Nature Communications, 7 . doi:10.1038/ncomms12976


Author Wang, Tong
Martin, Sally
Nguyen, Tam H.
Harper, Callista B.
Gormal, Rachel S.
Martinez-Marmol, Ramon
Karunanithi, Shanker
Coulson, Elizabeth J.
Glass, Nick R.
Cooper-White, Justin J.
Van Swinderen, Bruno
Meunier, Frederic A.
Title Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2016-09-30
Sub-type Article (original research)
DOI 10.1038/ncomms12976
Open Access Status DOI
Volume 7
Total pages 15
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Formatted abstract
Axonal retrograde transport of signalling endosomes from the nerve terminal to the soma underpins survival. As each signalling endosome carries a quantal amount of activated receptors, we hypothesized that it is the frequency of endosomes reaching the soma that determines the scale of the trophic signal. Here we show that upregulating synaptic activity markedly increased the flux of plasma membrane-derived retrograde endosomes (labelled using cholera toxin subunit-B: CTB) in hippocampal neurons cultured in microfluidic devices, and live Drosophila larval motor neurons. Electron and super-resolution microscopy analyses revealed that the fast-moving sub-diffraction-limited CTB carriers contained the TrkB neurotrophin receptor, transiently activated by synaptic activity in a BDNF-independent manner. Pharmacological and genetic inhibition of TrkB activation selectively prevented the coupling between synaptic activity and the retrograde flux of signalling endosomes. TrkB activity therefore controls the encoding of synaptic activity experienced by nerve terminals, digitalized as the flux of retrogradely transported signalling endosomes.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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