Cerebral microcirculation during mild head injury after a contusion and acceleration experimental model in sheep

Bellapart, Judith, Abi-Fares, Catherine, Cuthbertson, Kylie, Dunster, Kimble, Diab, Sara, Platts, David G., Raffel, Christopher, Gabrielian, Levon, Barnett, Adrian, Paratz, Jennifer, Boots, Rob and Fraser, John F. (2016) Cerebral microcirculation during mild head injury after a contusion and acceleration experimental model in sheep. Brain Injury, 1-10. doi:10.1080/02699052.2016.1199894


Author Bellapart, Judith
Abi-Fares, Catherine
Cuthbertson, Kylie
Dunster, Kimble
Diab, Sara
Platts, David G.
Raffel, Christopher
Gabrielian, Levon
Barnett, Adrian
Paratz, Jennifer
Boots, Rob
Fraser, John F.
Title Cerebral microcirculation during mild head injury after a contusion and acceleration experimental model in sheep
Journal name Brain Injury   Check publisher's open access policy
ISSN 1362-301X
0269-9052
Publication date 2016-08-26
Sub-type Article (original research)
DOI 10.1080/02699052.2016.1199894
Open Access Status Not yet assessed
Start page 1
End page 10
Total pages 10
Place of publication Abingdon, Oxfordshire, United Kingdom
Publisher Taylor & Francis
Collection year 2017
Language eng
Formatted abstract
Background: Cerebral microcirculation after head injury is heterogeneous and temporally variable. Regions at risk of infarction such as peri-contusional areas are vulnerable to anaemia. However, direct quantification of the cerebral microcirculation is clinically not feasible. This study describes a novel experimental head injury model correlating cerebral microcirculation with histopathology analysis.

Objective: To test the hypothesis that cerebral microcirculation at the ischaemic penumbrae is reduced over time when compared with non-injured regions.

Methods: Merino sheep were instrumented using a transeptal catheter to inject coded microspheres into the left cardiac atrium, ensuring systemic distribution. After a blunt impact over the left parietal region, cytometric analyses quantified cerebral microcirculation and amyloid precursor protein staining identified axonal injury in pre-defined anatomical regions. A mixed effect regression model assessed the hourly blood flow results during 4 hours after injury.

Results: Cerebral microcirculation showed temporal reductions with minimal amyloid staining except for the ipsilateral thalamus and medulla.

Conclusion: The spatial heterogeneity and temporal reduction of cerebral microcirculation in ovine models occur early, even after mild head injury, independent of the intracranial pressure and the level of haemoglobin. Alternate approaches to ensure recovery of regions with reversible injury require a targeted assessment of cerebral microcirculation.
Keyword Axonal damage
Brain trauma
Cerebral blood flow
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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