Plasma exosome profiles from dairy cows with divergent fertility phenotypes

Mitchell, M. D., Scholz-Romero, K., Reed, S., Peiris, H. N., Koh, Y. Q., Meier, S., Walker, C. G., Burke, C. R., Roche, J. R., Rice, G. and Salomon, C. (2016) Plasma exosome profiles from dairy cows with divergent fertility phenotypes. Journal of Dairy Science, 99 9: 7590-7601. doi:10.3168/jds.2016-11060


Author Mitchell, M. D.
Scholz-Romero, K.
Reed, S.
Peiris, H. N.
Koh, Y. Q.
Meier, S.
Walker, C. G.
Burke, C. R.
Roche, J. R.
Rice, G.
Salomon, C.
Title Plasma exosome profiles from dairy cows with divergent fertility phenotypes
Journal name Journal of Dairy Science   Check publisher's open access policy
ISSN 1525-3198
0022-0302
Publication date 2016-09-01
Year available 2016
Sub-type Article (original research)
DOI 10.3168/jds.2016-11060
Open Access Status Not yet assessed
Volume 99
Issue 9
Start page 7590
End page 7601
Total pages 12
Place of publication New York, NY United States
Publisher Elsevier
Collection year 2017
Language eng
Abstract Cell-to-cell communication in physiological and pathological conditions may be influenced by neighboring cells, distant tissues, or local environmental factors. Exosomes are specific subsets of extracellular vesicles that internalize and deliver their content to near and distant sites. Exosomes may play a role in the maternal-embryo crosstalk vital for the recognition and maintenance of a pregnancy; however, their role in dairy cow reproduction has not been established. This study aimed to characterize the exosome profile in the plasma of 2 strains of dairy cow with divergent fertility phenotypes. Plasma was obtained and characterized on the basis of genetic ancestry as fertile (FERT; <23% North American genetics, New Zealand Holstein-Friesian strain, n = 8) or subfertile (SUBFERT; >92% North American genetics, North American Holstein-Friesian strain, n = 8). Exosomes were isolated by differential and buoyant density centrifugation and characterized by size distribution (nanoparticle tracking analysis, NanoSight NS500, NanoSight Ltd., Amesbury, UK), the presence of CD63 (Western blot), and their morphology (electron microscopy). The total number of exosomes was determined by quantifying the immunoreactive CD63 (ExoELISA kit, System Biosciences), and the protein content established by mass spectrometry. Enriched exosome fractions were identified as cup-shape vesicles with diameters around 100 nm and positive for the CD63 marker. The concentration of exosomes was 50% greater in FERT cows. Mass spectrometry identified 104 and 117 proteins in FERT and SUBFERT cows, of which 23 and 36 were unique, respectively. Gene ontology analysis revealed enrichment for proteins involved in immunomodulatory processes and cell-to-cell communication. Although the role of exosomes in dairy cow reproduction remains to be elucidated, their quantification and content in models with divergent fertility phenotypes could provide novel information to support both physiological and genetic approaches to improving dairy cow fertility.
Keyword Exosomes
Fertility
Dairy cow
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
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