Pivotal role of the β-clamp in translesion DNA synthesis and mutagenesis in E. coli cells

Becherel, Oliver J., Fuchs, Robert P. P. and Wagner, Jerome (2002) Pivotal role of the β-clamp in translesion DNA synthesis and mutagenesis in E. coli cells. DNA Repair, 1 9: 703-708. doi:10.1016/S1568-7864(02)00106-4


Author Becherel, Oliver J.
Fuchs, Robert P. P.
Wagner, Jerome
Title Pivotal role of the β-clamp in translesion DNA synthesis and mutagenesis in E. coli cells
Formatted title
Pivotal role of the β-clamp in translesion DNA synthesis and mutagenesis in E. coli cells
Journal name DNA Repair   Check publisher's open access policy
ISSN 1568-7864
1568-7856
Publication date 2002-09-04
Sub-type Article (original research)
DOI 10.1016/S1568-7864(02)00106-4
Open Access Status Not yet assessed
Volume 1
Issue 9
Start page 703
End page 708
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
The genetic information is continuously subjected to the attack by endogenous and exogenous chemical and physical carcinogens that damage the DNA template, thus compromising its biochemical functions. Despite the multiple and efficient DNA repair systems that have evolved to cope with the large variety of damages, some lesions may persist and, as a consequence, interfere with DNA replication. By essence, the damaged-DNA replication process (hereafter termed translesion synthesis or TLS) is a major source of point mutations and is therefore deeply involved in the onset of human diseases such as cancer. Recent identification of numerous DNA polymerases involved in TLS has shed new light onto the molecular mechanisms of mutagenesis. Here, we show that in vivo, both error-free and mutagenic bypass activities of the three DNA polymerases known to be involved in TLS in Escherichia coli (PolII, PolIV and PolV) strictly depend upon the integrity of small peptidic sequences identified as their β-clamp binding motif. Thus, in addition to its crucial role as the processivity factor of the PolIII replicase, the β-clamp plays a pivotal role during the TLS process.
Keyword DNA polymerases
Escherichia coli
Mutagenesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Tue, 09 Aug 2016, 16:15:50 EST by Olivier Becherel on behalf of Learning and Research Services (UQ Library)