Mechanism of DNA polymerase II-mediated frameshift mutagenesis

Becherel, Olivier J. and Fuchs, Robert P. P. (2001) Mechanism of DNA polymerase II-mediated frameshift mutagenesis. National Academy of Sciences. Proceedings, 98 15: 8566-8571. doi:10.1073/pnas.141113398


Author Becherel, Olivier J.
Fuchs, Robert P. P.
Title Mechanism of DNA polymerase II-mediated frameshift mutagenesis
Journal name National Academy of Sciences. Proceedings   Check publisher's open access policy
ISSN 0027-8424
1091-6490
Publication date 2001-07-17
Sub-type Article (original research)
DOI 10.1073/pnas.141113398
Open Access Status Not yet assessed
Volume 98
Issue 15
Start page 8566
End page 8571
Total pages 6
Place of publication Washington, DC, United States
Publisher National Academy of Sciences
Language eng
Formatted abstract
Escherichia coli possesses three SOS-inducible DNA polymerases (Pol II, IV, and V) that were recently found to participate in translesion synthesis and mutagenesis. Involvement of these polymerases appears to depend on the nature of the lesion and its local sequence context, as illustrated by the bypass of a single N-2-acetylaminofluorene adduct within the Narl mutation hot spot. Indeed, error-free bypass requires Pol V (umuDC), whereas mutagenic (-2 frameshift) bypass depends on Pol II (polB). In this paper, we show that purified DNA Pol II is able in vitro to generate the -2 frameshift bypass product observed in vivo at the Narl sites. Although the ΔpolB strain is completely defective in this mutation pathway, introduction of the polB gene on a low copy number plasmid restores the -2 frameshift pathway. In fact, modification of the relative copy number of polB versus umuDC genes results in a corresponding modification in the use of the frameshift versus error-free translesion pathways, suggesting a direct competition between Pol II and V for the bypass of the same lesion. Whether such a polymerase competition model for translesion synthesis will prove to be generally applicable remains to be confirmed.
Keyword N-2-acetylaminofluorene
Narl mutation hot spot
Slippage mutagenesis
Translesion synthesis
UmuDC (Pol V)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Centre for Clinical Research Publications
 
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Created: Tue, 09 Aug 2016, 16:14:41 EST by Olivier Becherel on behalf of Learning and Research Services (UQ Library)