Kinetic characteristics and regulatory mechanisms of glucokinase and fructokinase from Zymomonas mobilis

Doelle H.W. (1982) Kinetic characteristics and regulatory mechanisms of glucokinase and fructokinase from Zymomonas mobilis. European Journal of Applied Microbiology and Biotechnology, 14 4: 241-246. doi:10.1007/BF00498471


Author Doelle H.W.
Title Kinetic characteristics and regulatory mechanisms of glucokinase and fructokinase from Zymomonas mobilis
Journal name European Journal of Applied Microbiology and Biotechnology
ISSN 0171-1741
Publication date 1982
Sub-type Article (original research)
DOI 10.1007/BF00498471
Volume 14
Issue 4
Start page 241
End page 246
Total pages 6
Publisher Springer-Verlag
Subject 2404 Microbiology
1305 Biotechnology
2700 Medicine
Abstract Glucokinase (EC 2.7.1.2) exhibits two pH optima (pH 7.0 and 8.2), gives hyperbolic saturation curves and reacts equally with ATP, UTP, GTP, ITP and CTP. Inhibition occurs with high concentrations of these nucleotides and in addition with ADP, AMP and glucose 6-phosphate. No inhibition was observed with sucrose, glucose, fructose (11 mM), ethanol (542 mM), mannose, ribose, galactose, deoxy-glucose, lactose and gluconate and no reaction except with glucose. Fructokinase (EC 2.7.1.4) exhibits one pH optimum (7.4), gives hyperbolic saturation curves and is highly specific towards ATP and fructose. Mannose, glucose, GTP and CTP do not react. Inhibition occurs with glucose, glucose 6-phosphate and mildly fructose 6-phosphate. ATP at high concentrations gives slight inhibition, ADP and AMP show differential effects, whereas all other above mentioned compounds do not inhibit. Regulatory mechanisms for sucrose, glucose and fructose metabolism are discussed.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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