The importance of electrostatic interactions in the binding of paraquat to its elicited monoclonal antibody

Bowles M.R. and Pond S.M. (1990) The importance of electrostatic interactions in the binding of paraquat to its elicited monoclonal antibody. Molecular Immunology, 27 9: 847-852. doi:10.1016/0161-5890(90)90150-X


Author Bowles M.R.
Pond S.M.
Title The importance of electrostatic interactions in the binding of paraquat to its elicited monoclonal antibody
Journal name Molecular Immunology   Check publisher's open access policy
ISSN 0161-5890
Publication date 1990
Sub-type Article (original research)
DOI 10.1016/0161-5890(90)90150-X
Volume 27
Issue 9
Start page 847
End page 852
Total pages 6
Subject 1312 Molecular Biology
2403 Immunology
Abstract In this study the pH-dependent interactions interactions between a paraquat-specific murine monoclonal antibody and two antigens: paraquat (1,1'-dimethyl-4,4'-bipyridinium dichloride), and a p-nitrophenol analogue (1-(N-methyl-4,4'-bipyridinium)-1-(2-hydroxy-5-nitrophenyl)methane dichloride; PQNP) were determined by ELISA. In each case the KAS-pH profile reflected the titration of a single amino acid residue. pK'a values derived from these plots were 8.90 (paraquat) and 8.13 (PQNP). Increasing pH led to a significant increase in the association constant for each antibody complex. A spectrophotometric titration of PQNP in the presence and absence of excess antibody indicated the presence of another charged amino acid residue at the binding site, which could be assigned as a carboxylic acid. From these studies, a model for paraquat-antibody binding has been proposed.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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