Identification and characterisation of the extracellular sucrases of Zymomonas mobilis UQM 2716 (ATCC 39676)

Crittenden R.G. and Doelle H.W. (1994) Identification and characterisation of the extracellular sucrases of Zymomonas mobilis UQM 2716 (ATCC 39676). Applied Microbiology and Biotechnology, 41 3: 302-308. doi:10.1007/BF00221223


Author Crittenden R.G.
Doelle H.W.
Title Identification and characterisation of the extracellular sucrases of Zymomonas mobilis UQM 2716 (ATCC 39676)
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
Publication date 1994
Sub-type Article (original research)
DOI 10.1007/BF00221223
Volume 41
Issue 3
Start page 302
End page 308
Total pages 7
Publisher Springer-Verlag
Subject 2726 Microbiology (medical)
2404 Microbiology
1502 Banking, Finance and Investment
1305 Biotechnology
Abstract An investigation was conducted to isolate, and characterise the extracellular sucrases of Zymomonas mobilis UQM 2716. Levansucrase (EC 2.4.1.10) was the only extracellular sucrase produced by this organism. This enzyme was responsible for sucrose hydrolysis, levan formation, and oligosaccharide production. It had a molecular mass of 98 kDa, a Michaelis constant (Km) of 64 m m, and a pH optimum of 5.5. It was inhibited by glucose, but not by fructose, ethanol, sorbitol, NaCl, TRIS or ethylenediaminetetraacetic acid (EDTA). The formation of levan was the principal reaction catalysed by this enzyme at low temperatures. However, levan formation was thermolabile, being irreversibly lost when levansucrase was heated to 35°C. S This did not effect sucrose hydrolysis or oligosaccharide formation, which were optimal at 45°C. Sucrose concentration greatly influenced the type of acceptor molecule used in the transfructosylation reactions catalysed by levansucrase. At low sucrose concentration, the predominant reaction catalysed was the hydrolysis of sucrose to free glucose and fructose. At high sucrose concentrations, oligosaccharide production was the major reaction catalysed.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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