Periodontal regeneration with stem cells-seeded collagen-hydroxyapatite scaffold

Liu, Zeping, Yin, Xing, Ye, Qingsong, He, Wulin, Ge, Mengke, Zhou, Xiaofu, Hu, Jing and Zou, Shujuan (2016) Periodontal regeneration with stem cells-seeded collagen-hydroxyapatite scaffold. Journal of Biomaterials Applications, 31 1: 121-131. doi:10.1177/0885328216637978


Author Liu, Zeping
Yin, Xing
Ye, Qingsong
He, Wulin
Ge, Mengke
Zhou, Xiaofu
Hu, Jing
Zou, Shujuan
Title Periodontal regeneration with stem cells-seeded collagen-hydroxyapatite scaffold
Journal name Journal of Biomaterials Applications   Check publisher's open access policy
ISSN 1530-8022
0885-3282
Publication date 2016-07-01
Year available 2016
Sub-type Article (original research)
DOI 10.1177/0885328216637978
Open Access Status Not yet assessed
Volume 31
Issue 1
Start page 121
End page 131
Total pages 11
Place of publication London, United Kingdom
Publisher Sage Publications
Collection year 2017
Language eng
Abstract Re-establishing compromised periodontium to its original structure, properties and function is demanding, but also challenging, for successful orthodontic treatment. In this study, the periodontal regeneration capability of collagen-hydroxyapatite scaffolds, seeded with bone marrow stem cells, was investigated in a canine labial alveolar bone defect model. Bone marrow stem cells were isolated, expanded and characterized. Porous collagen-hydroxyapatite scaffold and cross-linked collagen-hydroxyapatite scaffold were prepared. Attachment, migration, proliferation and morphology of bone marrow stem cells, co-cultured with porous collagen-hydroxyapatite or cross-linked collagen-hydroxyapatite, were evaluated in vitro. The periodontal regeneration capability of collagen-hydroxyapatite scaffold with or without bone marrow stem cells was tested in six beagle dogs, with each dog carrying one sham-operated site as healthy control, and three labial alveolar bone defects untreated to allow natural healing, treated with bone marrow stem cells - collagen-hydroxyapatite scaffold implant or collagen-hydroxyapatite scaffold implant, respectively. Animals were euthanized at 3 and 6 months (3 animals per group) after implantation and the resected maxillary and mandibular segments were examined using micro-computed tomography scan, H&E staining, Masson's staining and histometric evaluation. Bone marrow stem cells were successfully isolated and demonstrated self-renewal and multi-potency in vitro. The porous collagen-hydroxyapatite and cross-linked collagen-hydroxyapatite had average pore sizes of 415 ± 20 μm and 203 ± 18 μm and porosity of 69 ± 0.5% and 50 ± 0.2%, respectively. The attachment, proliferation and migration of bone marrow stem cells were satisfactory on both porous collagen-hydroxyapatite and cross-linked collagen-hydroxyapatite scaffolds. Implantation of bone marrow stem cells - collagen-hydroxyapatite or collagen-hydroxyapatite scaffold in beagle dogs with experimental periodontal defects resulted in significantly enhanced periodontal regeneration characterized by formation of new bone, periodontal ligament and cementum, compared with the untreated defects, as evidenced by histological and micro-computed tomography examinations. The prepared collagen-hydroxyapatite scaffolds possess favorable bio-compatibility. The bone marrow stem cells - collagen-hydroxyapatite and collagen-hydroxyapatite scaffold - induced periodontal regeneration, with no aberrant events complicating the regenerative process. Further research is necessary to improve the bone marrow stem cells behavior in collagen-hydroxyapatite scaffolds after implantation.
Keyword Bone marrow stem cells
Collagen-hydroxyapatite
Composite scaffold
Micro-computed tomography
Periodontal regeneration
Tissue engineering
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Dentistry Publications
 
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