Identification of a female spawn-associated Kazal-type inhibitor from the tropical abalone Haliotis asinina

Wang, Tianfang, Nuurai, Parinyaporn, McDougall, Carmel, York, Patrick S., Bose, Utpal, Degnan, Bernard M. and Cummins, Scott F. (2016) Identification of a female spawn-associated Kazal-type inhibitor from the tropical abalone Haliotis asinina. Journal of Peptide Science, 22 7: 461-470. doi:10.1002/psc.2887


Author Wang, Tianfang
Nuurai, Parinyaporn
McDougall, Carmel
York, Patrick S.
Bose, Utpal
Degnan, Bernard M.
Cummins, Scott F.
Title Identification of a female spawn-associated Kazal-type inhibitor from the tropical abalone Haliotis asinina
Formatted title
Identification of a female spawn-associated Kazal-type inhibitor from the tropical abalone Haliotis asinina
Journal name Journal of Peptide Science   Check publisher's open access policy
ISSN 1099-1387
1075-2617
Publication date 2016-07-01
Year available 2016
Sub-type Article (original research)
DOI 10.1002/psc.2887
Open Access Status Not Open Access
Volume 22
Issue 7
Start page 461
End page 470
Total pages 10
Place of publication Chichester, West Sussex, United Kingdom
Publisher John Wiley & Sons
Collection year 2017
Language eng
Formatted abstract
Abalone (Haliotis) undergoes a period of reproductive maturation, followed by the synchronous release of gametes, called broadcast spawning. Field and laboratory studies have shown that the tropical species Haliotis asinina undergoes a two-week spawning cycle, thus providing an excellent opportunity to investigate the presence of endogenous spawning-associated peptides. In female H. asinina, we have isolated a peptide (5145 Da) whose relative abundance in hemolymph increases substantially just prior to spawning and is still detected using reverse-phase high-performance liquid chromatography chromatograms up to 1-day post-spawn. We have isolated this peptide from female hemolymph as well as samples prepared from the gravid female gonad, and demonstrated through comparative sequence analysis that it contains features characteristic of Kazal-type proteinase inhibitors (KPIs). Has-KPI is expressed specifically within the gonad of adult females. A recombinant Has-KPI was generated using a yeast expression system. The recombinant Has-KPI does not induce premature spawning of female H. asinina when administered intramuscularly. However it displays homomeric aggregations and interaction with at least one mollusc-type neuropeptide (LRDFVamide), suggesting a role for it in regulating neuropeptide endocrine communication. This research provides new understanding of a peptide that can regulate reproductive processes in female abalone, which has the potential to lead to the development of greater control over abalone spawning. The findings also highlight the need to further explore abalone reproduction to clearly define a role for novel spawning-associated peptide in sexual maturation and spawning.
Keyword Abalone
Hemolymph
Ovary
Protease inhibitor
Reproduction
Spawning
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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School of Biological Sciences Publications
 
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