Metagenomics of rumen bacteriophage from thirteen lactating dairy cattle

Ross, Elizabeth M., Petrovski, Steve, Moate, Peter J. and Hayes, Ben J. (2013) Metagenomics of rumen bacteriophage from thirteen lactating dairy cattle. BMC Microbiology, 13 . doi:10.1186/1471-2180-13-242


Author Ross, Elizabeth M.
Petrovski, Steve
Moate, Peter J.
Hayes, Ben J.
Title Metagenomics of rumen bacteriophage from thirteen lactating dairy cattle
Journal name BMC Microbiology   Check publisher's open access policy
ISSN 1471-2180
Publication date 2013-11-01
Sub-type Article (original research)
DOI 10.1186/1471-2180-13-242
Open Access Status DOI
Volume 13
Total pages 11
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: The bovine rumen hosts a diverse and complex community of Eukarya, Bacteria, Archea and viruses (including bacteriophage). The rumen viral population (the rumen virome) has received little attention compared to the rumen microbial population (the rumen microbiome). We used massively parallel sequencing of virus like particles to investigate the diversity of the rumen virome in thirteen lactating Australian Holstein dairy cattle all housed in the same location, 12 of which were sampled on the same day.

Results: Fourteen putative viral sequence fragments over 30 Kbp in length were assembled and annotated. Many of the putative genes in the assembled contigs showed no homology to previously annotated genes, highlighting the large amount of work still required to fully annotate the functions encoded in viral genomes. The abundance of the contig sequences varied widely between animals, even though the cattle were of the same age, stage of lactation and fed the same diets. Additionally the twelve animals which were co-habited shared a number of their dominant viral contigs. We compared the functional characteristics of our bovine viromes with that of other viromes, as well as rumen microbiomes. At the functional level, we found strong similarities between all of the viral samples, which were highly distinct from the rumen microbiome samples.

Conclusions: Our findings suggest a large amount of between animal variation in the bovine rumen virome and that co-habiting animals may have more similar viromes than non co-habited animals. We report the deepest sequencing to date of the rumen virome. This work highlights the enormous amount of novelty and variation present in the rumen virome.
Keyword Virome
Rumen
Bacteriophage
Metagenomics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Article number 242

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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