A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA

Koo, Kevin M., Wee, Eugene J. H., Mainwaring, Paul N. and Trau, Matt (2016) A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA. Scientific reports, 6 . doi:10.1038/srep30722


Author Koo, Kevin M.
Wee, Eugene J. H.
Mainwaring, Paul N.
Trau, Matt
Title A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA
Journal name Scientific reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-07-29
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep30722
Open Access Status DOI
Volume 6
Total pages 6
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Formatted abstract
The TMPRSS2:ERG gene fusion is one of a series of highly promising prostate cancer (PCa) biomarker alternatives to the controversial serum PSA. Current methods for detecting TMPRSS2:ERG are limited in terms of long processing time, high cost and the need for specialized equipment. Thus, there is an unmet need for less complex, faster, and cheaper methods to enable gene fusion detection in the clinic. We describe herein a simple, rapid and inexpensive assay which combines robust isothermal
amplification technique with a novel visualization method for evaluating urinary TMPRSS2:ERG status at less than USD 5 and with minimal equipment. The assay is sensitive, and rapidly detects as low as 10copies of TMPRSS2:ERG transcripts while maintaining high levels of specificity.
Keyword Current prostate cancer (PCa) screening
Prostate-specific antigen (PSA) levels
Detection technique
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Fri, 05 Aug 2016, 09:50:31 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences