Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling

Baranski, M., Rourke, M., Loughnan, S., Hayes, B., Austin, C. and Robinson, N. (2008) Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling. Animal Genetics, 39 6: 606-614. doi:10.1111/j.1365-2052.2008.01773.x


Author Baranski, M.
Rourke, M.
Loughnan, S.
Hayes, B.
Austin, C.
Robinson, N.
Title Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling
Formatted title
Detection of QTL for growth rate in the blacklip abalone (Haliotis rubra Leach) using selective DNA pooling
Journal name Animal Genetics   Check publisher's open access policy
ISSN 0268-9146
1365-2052
Publication date 2008-12
Sub-type Article (original research)
DOI 10.1111/j.1365-2052.2008.01773.x
Open Access Status Not yet assessed
Volume 39
Issue 6
Start page 606
End page 614
Total pages 9
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
The objective of this study was to identify QTL for growth rate in the blacklip abalone Haliotis rubra using selective DNA pooling. Three full-sibling families of H. rubra derived from crosses of wild broodstock were used. DNA was extracted from the largest and smallest 10% of progeny and combined into two pools for each phenotypic tail. The DNA pools were typed with 139 microsatellites, and markers showing significant differences between the peak height ratios of alleles inherited from the parents were individually genotyped and analysed by interval mapping. A strong correlation (r = 0.94, P < 0.001) was found between the t-values from the analysis of pools and the t-values from the analysis of individual genotypes. Based on the interval mapping analysis, QTL were detected on nine linkage groups at a chromosome-wide P < 0.01 and one linkage group at a chromosome-wide P < 0.05. The study demonstrated that selective DNA pooling is efficient and effective as a first-pass screen for the discovery of QTL in an aquaculture species.
Keyword Abalone
Growth rate
Haliotis rubra
Quantitative trait loci
Selective DNA pooling
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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