Intravital multiphoton imaging of rhodamine 123 in the rat liver after intravenous dosing

Liu, Xin, Thorling, Camilla A., Jin, Lu and Roberts, Michael S. (2012) Intravital multiphoton imaging of rhodamine 123 in the rat liver after intravenous dosing. IntraVital, 1 1: 54-59. doi:10.4161/intv.21450


Author Liu, Xin
Thorling, Camilla A.
Jin, Lu
Roberts, Michael S.
Title Intravital multiphoton imaging of rhodamine 123 in the rat liver after intravenous dosing
Journal name IntraVital   Check publisher's open access policy
ISSN 2165-9087
Publication date 2012-07-01
Sub-type Article (original research)
DOI 10.4161/intv.21450
Open Access Status Not Open Access
Volume 1
Issue 1
Start page 54
End page 59
Total pages 6
Place of publication New York, United States
Publisher Taylor & Francis
Language eng
Abstract Intravital imaging with multiphoton microscopy was used to investigate the hepatic disposition of rhodamine 123 (RH123) in the exposed liver of anesthetized rats after intravenous dosing. The role played by the biliary canalicular transporter P-glycoprotein (P-gp) on the disposition of RH123 was explored by administering a P-gp inhibitor, cyclosporine A prior to RH123 administration. The fluorescence intensity of RH123 was defined by multiphoton microscopy using a femtosecond laser excitation wavelength of 900 nm whereas the autofluorescence at an excitation wavelength of 740 nm was used to define the morphology of the liver acini. Intravital imaging showed that RH123 was rapidly taken up from the sinusoids into hepatocytes but slowly eliminated from the cells (half-life 2.89 ± 1.37 h). The presence of cyclosporine A did not affect the uptake of RH123 but markedly increased the fluorescence intensity of RH123 in the liver and was associated with a slower elimination of RH123 from the liver (half-life 11.5 ± 4.24 h). In conclusion, the spatial disposition of RH123 in the rat liver and the effects of a transporter inhibitor on its disposition have been monitored over time using intravital imaging.
Keyword Drug delivery
In vivo imaging
Intravital microscopy
Multi-photon
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Thu, 28 Jul 2016, 13:58:05 EST by Camilla Thompson on behalf of Medicine - Princess Alexandra Hospital