Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy

Wang, Haolu, Liang, Xiaowen, Gravot, Germain, Thorling, Camilla A., Crawford, Darrell H. G., Xu, Zhi Ping, Liu, Xin and Roberts, Michael S. (2016) Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy. Journal of Biophotonics, 1-15. doi:10.1002/jbio.201600083


Author Wang, Haolu
Liang, Xiaowen
Gravot, Germain
Thorling, Camilla A.
Crawford, Darrell H. G.
Xu, Zhi Ping
Liu, Xin
Roberts, Michael S.
Title Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy
Journal name Journal of Biophotonics   Check publisher's open access policy
ISSN 1864-063X
1864-0648
Publication date 2016-06-17
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1002/jbio.201600083
Open Access Status Not Open Access
Start page 1
End page 15
Total pages 15
Place of publication Weinheim, Germany
Publisher Wiley - VCH
Collection year 2017
Language eng
Formatted abstract
Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases.
Keyword Multiphoton microscopy
Fluorescence lifetime imaging
Liver
Morphology
Function
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Early view

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: HERDC Pre-Audit
Admin Only - School of Medicine
School of Medicine Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Thu, 28 Jul 2016, 13:55:17 EST by Camilla Thompson on behalf of Medicine - Princess Alexandra Hospital