Using multiphoton fluorescence lifetime imaging to characterize liver damage and fluorescein disposition in liver in vivo

Thorling, Camilla A., Studier, Hauke, Crawford, Darrell H. G. and Roberts, Michael S. (2016). Using multiphoton fluorescence lifetime imaging to characterize liver damage and fluorescein disposition in liver in vivo. In: Ammasi Periasamy, Peter T. C. So and Karsten König, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Multiphoton Microscopy in the Biomedical Sciences XVI, San Francisco, CA, United States, (97120Y-1-97120Y-12). 14-16 February 2016. doi:10.1117/12.2235828


Author Thorling, Camilla A.
Studier, Hauke
Crawford, Darrell H. G.
Roberts, Michael S.
Title of paper Using multiphoton fluorescence lifetime imaging to characterize liver damage and fluorescein disposition in liver in vivo
Formatted title
Using multiphoton fluorescence lifetime imaging to characterize liver damage and fluorescein disposition in liver in vivo
Conference name Multiphoton Microscopy in the Biomedical Sciences XVI
Conference location San Francisco, CA, United States
Conference dates 14-16 February 2016
Proceedings title Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal name Progress in Biomedical Optics and Imaging
Place of Publication Bellingham, WA, United States
Publisher S P I E - International Society for Optical Engineering
Publication Year 2016
Sub-type Fully published paper
DOI 10.1117/12.2235828
Open Access Status Not Open Access
ISBN 9781628419467
ISSN 1605-7422
Editor Ammasi Periasamy
Peter T. C. So
Karsten König
Volume 9712
Start page 97120Y-1
End page 97120Y-12
Total pages 12
Chapter number 14
Total chapters 34
Collection year 2017
Language eng
Abstract/Summary Liver disease is the fifth most common cause of death and unlike many other major causes of mortality, liver disease rates are increasing rather than decreasing. There is no ideal measurement of liver disease and although biopsies are the gold standard, this only allows for a spot examination and cannot follow dynamic processes of the liver. Intravital imaging has the potential to extract detailed information over a larger sampling area continuously. The aim of this project was to investigate whether multiphoton and fluorescence lifetime imaging microscopy could detect early liver damage and to assess whether it could detect changes in metabolism of fluorescein in normal and diseased livers. Four experimental groups were used in this study: 1) control; 2) ischemia reperfusion injury; 3) steatosis and 4) steatosis with ischemia reperfusion injury. Results showed that multiphoton microscopy could visualize morphological changes such as decreased fluorescence of endogenous fluorophores and the presence of lipid droplets, characteristic of steatosis. Fluorescence lifetime imaging microscopy showed increase in NADPH in steatosis with and without ischemia reperfusion injury and could detect changes in metabolism of fluorescein to fluorescein monoglurcuronide, which was impaired in steatosis with ischemia reperfusion injury. These results concluded that the combination of multiphoton microscopy and fluorescence lifetime imaging is a promising method of assessing early stage liver damage and that it can be used to study changes in drug metabolism in the liver as an indication of liver disease and has the potential to replace the traditional static liver biopsy currently used.
Keyword Multiphoton microscopy
Fluorescence lifetime imaging
Liver
Fluorescein,
Ischemia reperfusion injury
Steatosis
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes The papers in this volume were part of the technical conference cited on the cover and title page. Papers were selected and subject to review by the editors and conference program committee.

 
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Created: Thu, 28 Jul 2016, 13:52:33 EST by Camilla Thompson on behalf of Medicine - Princess Alexandra Hospital