Interactions between T and B lymphocytes and macrophages in the production of leukocyte adherence inhibition factor

Dunn I.S. and Halliday W.J. (1980) Interactions between T and B lymphocytes and macrophages in the production of leukocyte adherence inhibition factor. Cellular Immunology, 52 1: 48-61. doi:10.1016/0008-8749(80)90399-8


Author Dunn I.S.
Halliday W.J.
Title Interactions between T and B lymphocytes and macrophages in the production of leukocyte adherence inhibition factor
Journal name Cellular Immunology   Check publisher's open access policy
ISSN 1090-2163
Publication date 1980
Sub-type Article (original research)
DOI 10.1016/0008-8749(80)90399-8
Volume 52
Issue 1
Start page 48
End page 61
Total pages 14
Subject 1307 Cell Biology
2403 Immunology
Abstract The mechanism of the leukocyte adherence inhibition (LAI) reaction was investigated using CBA mice sensitized to picryl chloride. A trinitrophenylated conjugate of bovine serum albumin, used as in vitro antigen, gave a sharp peak of reactivity in dose-response studies using spleen cells prepared 7 days after sensitization. Supernatants prepared from antigen-treated spleen cells contained a leukocyte adherence inhibition factor (LAIF), which inhibited adherence of normal peritoneal cells (PC) in two-stage (indirect) reactions, although it was difficult to demonstrate LAIF production reproducibly with unfractionated spleen cells. Macrophage depletion of sensitized cells was effected by passage through glass beads or relatively rapid passage through nylon wool. The former treatment abrogated LAIF production, indicating a macrophage requirement. Cells depleted by treatment with glass beads (but not cells treated with nylon wool) could have their LAIF-producing activity restored by normal PC; this occurred even with those cells which failed to produce detectable LAIF before depletion. It was inferred that a glass-adherent suppressor cell was responsible for obscuring initial activity, especially later than 7 days after sensitization. Ig+(B) and Ig-(T) lymphocytes were separated on a rabbit anti-mouse F(ab′)2 affinity column, and both populations produced LAIF when stimulated by antigen, provided normal macrophages (as PC) were also present. Treatment of these populations with anti-Thy-1.2 serum abrogated T-cell activity, whereas B-cell activity was unaffected. Sensitized B and T lymphocytes exerted a mutually suppressive effect when mixed together; this interaction may also contribute to inital nonreactivity of unfractionated cells. This paper presents evidence for a complex LAI reaction with spleen cells from mice sensitized to picryl chloride (PCl). The mechanism involves production of a soluble leukocyte adherence inhibition factor (LAIF) by both T and B lymphocytes in the presence of accessory cells. Suppressive effects between T and B cells in terms of LAI reactivity are also evident.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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