Efficient transformation with regeneration of the tropical pasture legume Stylosanthes humilis using Agrobacterium rhizogenes and a Ti plasmid-binary vector system

Manners J.M. and Way H. (1989) Efficient transformation with regeneration of the tropical pasture legume Stylosanthes humilis using Agrobacterium rhizogenes and a Ti plasmid-binary vector system. Plant Cell Reports, 8 6: 341-345. doi:10.1007/BF00716669


Author Manners J.M.
Way H.
Title Efficient transformation with regeneration of the tropical pasture legume Stylosanthes humilis using Agrobacterium rhizogenes and a Ti plasmid-binary vector system
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 1989
Sub-type Article (original research)
DOI 10.1007/BF00716669
Volume 8
Issue 6
Start page 341
End page 345
Total pages 5
Publisher Springer-Verlag
Subject 1110 Nursing
Abstract Agrobacterium rhizogenes carrying the binary Ti plasmid vector pGA492 was used to transform leaf and stem explants of the tropical pasture legume Stylosanthes humilis. Conditions which yielded kanamycin resistant roots at a frequency of up to 86% and subsequent plant regeneration at a frequency of 23% were defined. Transgenic plants were fertile and either grew normally or had stunted growth but otherwise showed only minor morphological abnormalities. Transgenic plants with normal phenotypes were obtained in the progeny of the primary regenerants. The presence of active neomycin phosphotransferase enzyme activity and binary vector DNA and TL-DNA was demonstrated in the regenerated plants. Evidence for the independent transfer of binary vector and TL-DNA was also obtained. This high frequency production of transgenic plants of S. humilis is a major improvement over previous methods using disarmed strains of A. tumefaciens as helper.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 26 Jul 2016, 05:05:42 EST by System User