The use of suspensions of isolated rat mucosal cells to study mechanisms of iron absorption

Halliday J.W. and Powell L.W. (1973) The use of suspensions of isolated rat mucosal cells to study mechanisms of iron absorption. Clinica Chimica Acta, 43 2: 267-276. doi:10.1016/0009-8981(73)90460-9


Author Halliday J.W.
Powell L.W.
Title The use of suspensions of isolated rat mucosal cells to study mechanisms of iron absorption
Journal name Clinica Chimica Acta   Check publisher's open access policy
ISSN 0009-8981
Publication date 1973-01-24
Sub-type Article (original research)
DOI 10.1016/0009-8981(73)90460-9
Volume 43
Issue 2
Start page 267
End page 276
Total pages 10
Subject 1303 Specialist Studies in Education
1308 Clinical Biochemistry
Abstract A method for the preparation of suspensions of isolated, viable intestinal cells has been modified to study the initial rapid phase of iron uptake and absorption by the cells. The technique permits precise timing of absorption studies and avoids contamination by plasma transferrin. Iron uptake by the cell suspensions showed an initial rapid phase in the first 2 min followed by a slower uptake to 30 min. Column chromatographic analysis of the 10000 g supernatant from the cell homogenates revealed 4 iron-containing fractions, one of which was ferritin. The others included a fraction of molecular weight higher than ferritin and another of molecular weight lower than 50000. The appearance of iron in these 2 peaks preceded iron incorporation into ferritin. The results suggest that these fractions represent cytoplasmic intermediates involved in the uptake and transport of iron by the mucosal cell. Ascorbic acid increased the uptake of iron by the cells and was necessary for the incorporation of iron into ferritin.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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