Calcium alginate immobilized hybridomas grown using a fluidized-bed perfusion system with a protein-free medium

Shen B., Greenfield P. and Reid S. (1994) Calcium alginate immobilized hybridomas grown using a fluidized-bed perfusion system with a protein-free medium. Cytotechnology, 14 2: 109-117. doi:10.1007/BF00758175


Author Shen B.
Greenfield P.
Reid S.
Title Calcium alginate immobilized hybridomas grown using a fluidized-bed perfusion system with a protein-free medium
Journal name Cytotechnology   Check publisher's open access policy
ISSN 0920-9069
Publication date 1994
Sub-type Article (original research)
DOI 10.1007/BF00758175
Volume 14
Issue 2
Start page 109
End page 117
Total pages 9
Publisher Kluwer Academic Publishers
Subject 1308 Clinical Biochemistry
1307 Cell Biology
1305 Biotechnology
Abstract Hybridoma SPO1 cells were immobilized in calcium alginate beads and were further grown in a fluidized-bed perfusion system with a protein-free medium. The presence of serum in the steps of entrapment was shown to be helpful for the preservation of cell viability. Each step during immobilization was investigated with respect to the extent of cell damage caused. The immobilization process using small beads caused a lower cell viability initially but allowed a higher rate of cell growth subsequently, compared to those in large beads. In a perfusion system for the continuous production of monoclonal antibodies (MAb), the viable cell density reached 2×107 cells per ml of beads with a viability of 40%. Compared with the cells in suspension culture, the immobilized SPO1 cells showed higher viable cell based specific rates of substrate uptake (glucose and glutamine) and of MAb production. A significant drop in the formation of lactate after the cell growth entered a steady state suggested a higher activity of the Tricarboxylic Acid Cycle in the cells when the cell density became high.
Keyword calcium alginate
fluidized-bed
hybridoma
metabolism
perfusion
protein-free medium
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 26 Jul 2016, 05:05:07 EST by System User