A technique for determining the shear sensitivity of mammalian cells in suspension culture

Smith C.G., Greenfield P.F. and Randerson D.H. (1987) A technique for determining the shear sensitivity of mammalian cells in suspension culture. Biotechnology Techniques, 1 1: 39-44. doi:10.1007/BF00156285


Author Smith C.G.
Greenfield P.F.
Randerson D.H.
Title A technique for determining the shear sensitivity of mammalian cells in suspension culture
Journal name Biotechnology Techniques   Check publisher's open access policy
ISSN 0951-208X
Publication date 1987
Sub-type Article (original research)
DOI 10.1007/BF00156285
Volume 1
Issue 1
Start page 39
End page 44
Total pages 6
Publisher Kluwer Academic Publishers
Subject 2402 Applied Microbiology and Biotechnology
1303 Specialist Studies in Education
Abstract A murine hybridoma was subjected to constant shear rates within a couette viscometer for periods of 15 hours. Shear effects on cells were determined by live cell counts, cell viability and by the release of the cytoplasmic enzyme lactate dehydrogenase into the culture medium. Cell damage was observed at a shear rate of 870s-1 but not at 420s-1.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 26 Jul 2016, 04:36:07 EST by System User