Stability and extractability of double-stranded RNA of pangola stunt and sugarcane Fiji disease viruses in dried plant tissues

Karan M., Hicks S., Harding R.M. and Teakle D.S. (1991) Stability and extractability of double-stranded RNA of pangola stunt and sugarcane Fiji disease viruses in dried plant tissues. Journal of Virological Methods, 33 1-2: 211-216. doi:10.1016/0166-0934(91)90020-Z


Author Karan M.
Hicks S.
Harding R.M.
Teakle D.S.
Title Stability and extractability of double-stranded RNA of pangola stunt and sugarcane Fiji disease viruses in dried plant tissues
Journal name Journal of Virological Methods   Check publisher's open access policy
ISSN 0166-0934
Publication date 1991
Sub-type Article (original research)
DOI 10.1016/0166-0934(91)90020-Z
Volume 33
Issue 1-2
Start page 211
End page 216
Total pages 6
Subject 2406 Virology
Abstract When leaves infected with pangola stunt virus (PaSV) were dried at 23, 37, 50, 70 or 105°C, the dsRNA was stable and could be extracted after aerobic storage at room temperature for 1 month, although at 105°C the amount obtained was reduced. The dsRNA was also recovered after leaves were freeze dried and stored in vacuo at room temperature for 6 months, or were dried and stored aerobically at room temperature for 10.5 months. dsRNA of sugarcane Fiji disease virus (FDV) was also stable when infected leaves were dried at 23, 37, 50 or 105°C and stored aerobically for 3 months or for at least 6 months when infected leaves were dried at 70°C. The unexpected high stability and extractability of both PaSV and FDV dsRNA when dried in leaves at low or high temperatures and stored at room temperature indicate that these, and probably other plant-infecting reoviruses, can be transported readily in desiccated host tissue between different countries for later extraction and comparison of their dsRNAs.
Keyword Double-stranded RNA
Dried leaf
Plant reovirus
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 2 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 26 Jul 2016, 04:07:56 EST by System User