Effect of ethanol on cholesterol and phospholipid composition of HeLa cells

Keegan R., Wilce P.A., Ruczkal-Pietrzak E. and Shanley B.C. (1983) Effect of ethanol on cholesterol and phospholipid composition of HeLa cells. Biochemical and Biophysical Research Communications, 114 3: 985-990. doi:10.1016/0006-291X(83)90657-5


Author Keegan R.
Wilce P.A.
Ruczkal-Pietrzak E.
Shanley B.C.
Title Effect of ethanol on cholesterol and phospholipid composition of HeLa cells
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1983-08-12
Sub-type Article (original research)
DOI 10.1016/0006-291X(83)90657-5
Volume 114
Issue 3
Start page 985
End page 990
Total pages 6
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Chronic exposure of animals to ethanol leads to changes in membrane lipid composition which may be related to the development of tolerance and physical dependence. The object of the present study was to investigate this phenomenon at a cellular level. HeLa cells were grown in the presence of ethanol (86 mM) for periods of up to 9 days. Both the cholesterol and phospholipid concentration of these cells increased during this period but the cholesterol: phospholipid ratio remained unchanged. Among the phosolipid classes phosphatidic acid decreased while phosphatidylethanolamine, phosphatidylcholine and phosphatidylserine increased rapidly, returning toward control values by 9 days. Significant decreases were observed in saturated (14:0, 16:0) and monoenoic (16:1, 18:1) fatty acids while the major polyenoic fatty acid (20:4) increased. It is concluded that cultured mammalian cells represent a useful model for investigation of the direct effects of ethanol on membrane lipid metabolism.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 26 Jul 2016, 04:05:04 EST by System User